Evidence map›Paper›PMID 30834072›Full record

ArticleJournal of extracellular vesicles2019

Subtypes of tumour cell-derived small extracellular vesicles having differently externalized phosphatidylserine.

Sachiko Matsumura, Tamiko Minamisawa, Kanako Suga, Hiromi Kishita, Takanori Akagi, Takanori Ichiki, Yuki Ichikawa, Kiyotaka Shiba

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of extracellular vesicles, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.

0numbers the graph read from it
0cells of the map it votes in
63citing papers in PubMed
4.8field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

63 citing papers in PubMed, 100 citations in OpenAlex.

  1. Article
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  4. Glucosylceramides trigger the shedding of alpha-synuclein laden ectosomes.Extracellular vesicles and circulating nucleic acids · 2026
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3 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Sachiko MatsumuraDivision of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan.
Tamiko MinamisawaDivision of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan.
Kanako SugaDivision of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan.
Hiromi KishitaDepartment of Material Engineering, School of Engineering, The University of Tokyo, Tokyo, Japan.
Takanori AkagiDepartment of Material Engineering, School of Engineering, The University of Tokyo, Tokyo, Japan.
Takanori IchikiDepartment of Material Engineering, School of Engineering, The University of Tokyo, Tokyo, Japan.
Yuki IchikawaIMRA America, Inc., Ann Arbor, MI, USA.
Kiyotaka ShibaDivision of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan.
Japanese Foundation For Cancer Research · JPThe University of Tokyo · JPAnn Arbor Center for Independent Living · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Phosphatidylserine (PS) has skewed distributions in the plasma membrane and is preferentially located in the inner leaflet of normal cells. Tumour cells, however, expose PS at the outer leaflet of cell surfaces, thereby potentially modulating the bio-signalling of cells. Interestingly, exosomes - or, more properly, small extracellular vesicles (sEVs) - which are secreted from tumour cells, are enriched with externalized PS, have been proposed as being involved in the progression of cancers, and could be used as a marker for tumour diagnostics. However, the sEV fractions prepared from various methods are composed of different subtypes of vesicles, and knowledge about the subtypes enriched with exposed PS is still limited. Here, we differentiated sEVs from cancer cell lines by density gradient centrifugation and characterized the separated fractions by using gold-labelling of PS in atomic force microscopy, thrombin generation assay, size and zeta potential measurements, and western blot analysis. These analyses revealed a previously unreported PS

Indexed as

Atomic force microscopydensity gradient centrifugationexosomesextracellular vesiclesisolationphosphatidylserinethrombin generation

Identifiers

PMID30834072
PMCPMC6394288
OpenAlexW2916210549

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.