Evidence map›Paper›PMID 30833691›Full record

ArticleNature nanotechnology2019

Bright sub-20-nm cathodoluminescent nanoprobes for electron microscopy.

Maxim B Prigozhin, Peter C Maurer, Alexandra M Courtis, Nian Liu, Michael D Wisser, Chris Siefe, Bining Tian, Emory Chan, Guosheng Song, Stefan Fischer and 11 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature nanotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
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  5. Spatial Multiplexing and Omics.Nature reviews. Methods primers · 2024
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

21 authors.

Maxim B PrigozhinDepartment of Physics, Stanford University, Stanford, CA, USA.
Peter C MaurerDepartment of Physics, Stanford University, Stanford, CA, USA.
Alexandra M CourtisDepartment of Chemistry, University of California at Berkeley, Berkeley, CA, USA.ORCID http://orcid.org/0000-0001-5602-507X
Nian LiuDepartment of Materials Science and Engineering, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-5966-0244
Michael D WisserDepartment of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
Chris SiefeDepartment of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
Bining TianMolecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Emory ChanMolecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Guosheng SongDepartment of Radiology, Stanford University, Stanford, CA, USA.
Stefan FischerDepartment of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
Shaul AloniMolecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
D Frank OgletreeMolecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Edward S BarnardMolecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.ORCID http://orcid.org/0000-0003-4736-0743
Lydia-Marie JoubertCSIF Beckman Center, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-4709-007X
Jianghong RaoDepartment of Radiology, Stanford University, Stanford, CA, USA.
A Paul AlivisatosDepartment of Chemistry, University of California at Berkeley, Berkeley, CA, USA.
Roger M MacfarlaneIBM Research - Almaden, San Jose, CA, USA.
Bruce E CohenMolecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.ORCID http://orcid.org/0000-0003-3655-3638
Yi CuiDepartment of Materials Science and Engineering, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-6103-6352
Jennifer A DionneDepartment of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
Steven ChuDepartment of Physics, Stanford University, Stanford, CA, USA. schu@stanford.edu.

Funding

Noninvasive deep-tissue single-cell imaging and nanoprobe developmentR01GM128089 · NIGMS · STANFORD UNIVERSITY · PI CHU, STEVEN, RAO, JIANGHONG · 2018 to 2021
$2.2M
NIGMS NIH HHS R01 GM128089
6 · The paper itself

Abstract

Electron microscopy has been instrumental in our understanding of complex biological systems. Although electron microscopy reveals cellular morphology with nanoscale resolution, it does not provide information on the location of different types of proteins. An electron-microscopy-based bioimaging technology capable of localizing individual proteins and resolving protein-protein interactions with respect to cellular ultrastructure would provide important insights into the molecular biology of a cell. Here, we synthesize small lanthanide-doped nanoparticles and measure the absolute photon emission rate of individual nanoparticles resulting from a given electron excitation flux (cathodoluminescence). Our results suggest that the optimization of nanoparticle composition, synthesis protocols and electron imaging conditions can lead to sub-20-nm nanolabels that would enable high signal-to-noise localization of individual biomolecules within a cellular context. In ensemble measurements, these labels exhibit narrow spectra of nine distinct colours, so the imaging of biomolecules in a multicolour electron microscopy modality may be possible.

Indexed as

Microscopy, Electron, TransmissionFluorescent DyesNanoparticlesFluorescent Dyes

Identifiers

PMID30833691
PMCPMC6786485

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.