ArticleScientific reports2019
In Vivo Flow Cytometry of Extremely Rare Circulating Cells.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- An immunocompetent, multifluorescent resource for real-time analysis of osteosarcoma progression, metastasis, and response to therapy.Science advances · 2026Article
- Multivalent Bi-Specific Nanoerythrosomes: A Two-Birds-One-Stone Approach to Combine Homologous and Active Targeting for Effective Thrombolysis.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Machine learning approach for enumeration of circulating cells with diffuseBiophotonics discovery · 2026Article
- Machine Learning Approach for Enumeration of Circulating Cells with DiffusebioRxiv : the preprint server for biology · 2026Article
- Considerations for the use of targeted fluorescence contrast agents to detect circulating cancer cell populations with diffuseJournal of biomedical optics · 2026Article
- Treatment of Acute Liver Injury through Selective Tropism ofACS nano · 2025Article
- In Vivo Labeling and Detection of Circulating Tumor Cells in Mice Using OTL38.Molecular imaging and biology · 2024Article
- Monitoring Circulating Myeloid Cells in Peritonitis with an In Vivo Imaging Flow Cytometer.Biomolecules · 2024Article
- Two-color diffuseJournal of biomedical optics · 2024Article
- Deep learning-enabled detection of rare circulating tumor cell clusters in whole blood using label-free, flow cytometry.Lab on a chip · 2024Article
- Article
- Fluorescent and Bioluminescent Reporter Mouse-Adapted Ebola Viruses Maintain Pathogenicity and Can Be Visualized in Vivo.The Journal of infectious diseases · 2023Article
- Ratiometric fluorescence sensing and quantification of circulating blood sodium sensors in miceBiomedical optics express · 2023Article
- High-Throughput Single-Cell Analysis of Nanoparticle-Cell Interactions.Trends in analytical chemistry : TRAC · 2023Article
- Deep Learning-Enabled, Detection of Rare Circulating Tumor Cell Clusters in Whole Blood Using Label-free, Flow Cytometry.bioRxiv : the preprint server for biology · 2023Article
- Article
- Dual-ratio approach for detection of point fluorophores in biological tissue.Journal of biomedical optics · 2023Article
- Review
- Near-infrared diffuse in vivo flow cytometry.Journal of biomedical optics · 2022Article
- Label-free flow cytometry of rare circulating tumor cell clusters in whole blood.Scientific reports · 2022Article
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Authors and funding
6 authors.
Funding
Abstract
Circulating tumor cells (CTCs) are of great interest in cancer research, but methods for their enumeration remain far from optimal. We developed a new small animal research tool called "Diffuse in vivo Flow Cytometry" (DiFC) for detecting extremely rare fluorescently-labeled circulating cells directly in the bloodstream. The technique exploits near-infrared diffuse photons to detect and count cells flowing in large superficial arteries and veins without drawing blood samples. DiFC uses custom-designed, dual fiber optic probes that are placed in contact with the skin surface approximately above a major vascular bundle. In combination with a novel signal processing algorithm, DiFC allows counting of individual cells moving in arterial or venous directions, as well as measurement of their speed and depth. We show that DiFC allows sampling of the entire circulating blood volume of a mouse in under 10 minutes, while maintaining a false alarm rate of 0.014 per minute. In practice, this means that DiFC allows reliable detection of circulating cells below 1 cell per mL. Hence, the unique capabilities of DiFC are highly suited to biological applications involving very rare cell types such as the study of hematogenous cancer metastasis.
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