ArticleScientific reports2019
Selection and Validation of Reference Genes for Gene Expression Studies by RT-PCR in Dalbergia odorifera.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Screening of Reference Gene for RT-qPCR inInternational journal of molecular sciences · 2026Article
- Insights into Variations in Chemical Profiles and Antioxidant Properties Among Different Parts ofPlants (Basel, Switzerland) · 2025Article
- Selection and validation of reference genes for quantitative real-time PCR during the developmental stages of seeds inFrontiers in plant science · 2025Article
- Dietary restriction reveals sex-specific expression of the mTOR pathway genes in Japanese quails.Scientific reports · 2024Article
- Changes in the physiological activity of parenchyma cells in Dalbergia odorifera xylem and its relationship with heartwood formation.BMC plant biology · 2023Article
- Function of the R2R3-MYB Transcription Factors inInternational journal of molecular sciences · 2023Article
- Selection and verification of reliable internal reference genes in stem development of herbaceous peony (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Genome-wide identification, characterization, and genetic diversity ofFrontiers in plant science · 2022Article
- Integrated mRNA and Small RNA Sequencing Reveals microRNAs Associated With Xylem Development inFrontiers in genetics · 2022Article
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4 authors.
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Abstract
Perennial tree Dalbergia odorifera T. Chen could form the precious heartwood used to produce chinese traditional medicine, rosewood furniture and fragrances. However the formation of heartwood is time-consuming and low efficient, leading to the severe destruction of its wild resources. Thus, it is urgent to study the molecular mechanism of heartwood formation in D. odorifera. But till now, there is no report about the reference gene selection in this species. In this study, the expression stability of nine candidate reference genes were evaluated across different tissues and stems treated by wound and chemical stimulators. Four algorithms were applied to obtain the robust genes. The results support HIS2, GAPDH, and CYP to be the most stable reference genes in samples under different wound treatments while DNAj was the least stable. In different tissues, HIS2, UBQ, and RPL were the most stable reference genes while DNAj was the least stable. The selected reference genes were validated through the normalization of the qRT-PCR data of six heartwood related genes in terpene biosynthesis pathway and ethylene signal pathway. The results showed that their expression levels were accurate when they were normalized by the most stable reference gene HIS2, or by the combination of the two or three most stable reference genes. These results demonstrated that these selected reference genes are reliable.
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