ArticlePloS one2019
Multiplex PCR methods for detection of several viruses associated with canine respiratory and enteric diseases.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- A systematic review and meta-analysis of canine enteric coronavirus prevalence in dogs of mainland China.Virology journal · 2024Pooled it
- Evaluation of a Point-of-Care Multiplex PCR Analyser for Detection ofVeterinary sciences · 2026Article
- Development of a multiplex polymerase chain reaction assay for the detection of Piroplasma (Open veterinary journal · 2026Article
- Fowl adenovirus infections: A comprehensive review of prevalence, pathogenesis, diagnosis, control, and economic impact.Poultry science · 2026Review
- First Molecular Insights into the Presence of Canine Kobuvirus in Ecuadorian Dogs Through the Standardization of a Sensitive SYBR Green RT-qPCR Assay.Veterinary sciences · 2025Article
- Novel multiplex family-wide PCR and Nanopore sequencing of amplicons (FP-NSA) approach for surveillance of influenza- and coronaviruses in humans and animals.Genome medicine · 2025Article
- Isolation and genetic characterization of parvovirus from Bengal tiger in China.Virology journal · 2025Article
- Stillbirth Caused by Vitamin A Deficiency in Captive African Lion Cubs (Panthera leo).Veterinary medicine and science · 2025Article
- Canine circovirus: an emerging virus of dogs and wild canids.Irish veterinary journal · 2025Review
- The Detection of Mixed Infection with Canine Parvovirus, Canine Distemper Virus, and Rotavirus in Giant Pandas by Multiplex PCR.Veterinary sciences · 2025Article
- Development of Multiplex PCR assay for detection of Canine Infectious Respiratory Disease Complex (CIRDC) pathogens in dogs.Frontiers in cellular and infection microbiology · 2025Article
- Automated highly multiplex detection system for respiratory pathogens in canines.Frontiers in veterinary science · 2025Article
- Development of one-step multiplex real-time PCR for the detection of CHV-1, CAdV-2, and CDV.Frontiers in veterinary science · 2025Article
- Canine circovirus: emergence, adaptation, and challenges for animal and public health.Frontiers in veterinary science · 2025Review
- Canine parvovirus in North-East India: a phylogenetic and evolutionary analysis.The veterinary quarterly · 2024Article
- Establishment of a Quadruplex RT-qPCR for the Detection of Canine Coronavirus, Canine Respiratory Coronavirus, Canine Adenovirus Type 2, and Canine Norovirus.Pathogens (Basel, Switzerland) · 2024Article
- Development of a Large-Scale Pathogen Screening Test for the Biosafety Evaluation of Canine Mesenchymal Stem Cells.Biological procedures online · 2023Article
- Preparation for the next pandemic: challenges in strengthening surveillance.Emerging microbes & infections · 2023Article
- Article
- An Improved Duplex Real-Time Quantitative RT-PCR Assay with a Canine Endogenous Internal Positive Control for More Sensitive and Reliable Detection of Canine Parainfluenza Virus 5.Veterinary sciences · 2023Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viral respiratory and intestinal infections are the most common causes of canine viral illness. Infection with multiple pathogens occurs in many cases. Rapid diagnosis of these multiple infections is important for providing timely and effective treatment. To improve diagnosis, in this study, two new multiplex polymerase chain reactions (mPCRs) were developed for simultaneous detection of canine respiratory viruses (CRV) and canine enteric viruses (CEV) using two separate primer mixes. The viruses included canine adenovirus type 2 (CAV-2), canine distemper virus (CDV), canine influenza virus (CIV), canine parainfluenza virus (CPIV), canine circovirus (CanineCV), canine coronavirus (CCoV) and canine parvovirus (CPV). The sensitivity of the mPCR results showed that the detection limit of both mPCR methods was 1×104 viral copies. Twenty nasal swabs (NS) and 20 anal swabs (AS) collected from dogs with symptoms of respiratory disease or enteric disease were evaluated using the novel mPCR methods as a clinical test. The mPCR protocols, when applied to these respiratory specimens and intestinal samples, could detect 7 viruses simultaneously, allowing rapid investigation of CRV (CAV-2, CDV, CIV and CPIV) and CEV (CAV-2, CanineCV, CCoV and CPV) status and prompt evaluation of coinfection. Our study provides an effective and accurate tool for rapid differential diagnosis and epidemiological surveillance in dogs.
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