ArticleACS chemical biology2019
Esterification Delivers a Functional Enzyme into a Human Cell.
Article in ACS chemical biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Protein Modifications for Cellular Protein Delivery.Chemical reviews · 2026Review
- Cell Penetrating Thyclotides Facilitate Efficient Delivery of Bioactive Peptides into Cells.bioRxiv : the preprint server for biology · 2026Article
- Reductive Methylation: An Alternative to Lysine → Arginine Mutagenesis.Journal of peptide science : an official publication of the European Peptide Society · 2026Article
- Pseudouridine residues as substrates for serum ribonucleases.RNA (New York, N.Y.) · 2025Article
- Hydrophilic α-Aryl-α-Diazoamides for Protein Esterification.Synlett : accounts and rapid communications in synthetic organic chemistry · 2025Article
- Carriers Multimerize STING Protein Fragments to Activate Type I Interferon Signaling in STING-Deficient Cancer Cells.Molecular pharmaceutics · 2025Article
- From Concepts to Inhibitors: A Blueprint for Targeting Protein-Protein Interactions.Chemical reviews · 2025Review
- Denitrogenative Alkylation of K-Ras(G12D) Inhibits Oncogenic Signaling in Cancer Cells.Journal of the American Chemical Society · 2025Article
- Chemoselective dual functionalization of proteins via 1,6-addition of thiols to trifunctional N-alkylpyridinium.Nature communications · 2025Article
- Multiprobe Photoproximity Labeling of the EGFR Interactome in Glioblastoma Using Red-Light.Journal of the American Chemical Society · 2025Article
- Mammalian Esterase Activity: Implications for Peptide Prodrugs.Biochemistry · 2024Article
- Chemoselective Reagents for the Traceless Bioreversible Modification of Native Proteins.Bioconjugate chemistry · 2024Article
- Unnatural lipids for simultaneous mRNA delivery and metabolic cell labeling.Biomaterials science · 2024Article
- Evaluation of the RNA-dependence of PRC2 binding to chromatin in human pluripotent stem cells.bioRxiv : the preprint server for biology · 2024Article
- Evaluation of the Cytosolic Uptake of HaloTag Using a pH-Sensitive Dye.ACS chemical biology · 2024Article
- Investigating the Cytosolic Delivery of Proteins by Lipid Nanoparticles Using the Chloroalkane Penetration Assay.Biochemistry · 2024Article
- Article
- Modular Diazo Compound for the Bioreversible Late-Stage Modification of Proteins.Journal of the American Chemical Society · 2023Article
- Hydrolysis-Resistant Ester-Based Linkers for Development of Activity-Based NIR Bioluminescence Probes.Journal of the American Chemical Society · 2023Article
- Host-Guest Complexation by β-Cyclodextrin Enhances the Solubility of an Esterified Protein.Molecular pharmaceutics · 2022Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
A major hurdle in chemical biology is the delivery of native proteins into the cytosol of mammalian cells. Herein, we report that esterification of the carboxyl groups of an enzyme with a diazo compound enables not only its passage into the cytosol but also the retention of its catalytic activity there. This scenario is demonstrated with human ribonuclease 1, which manifests ribonucleolytic activity that can be cytotoxic. After internalization, the nascent esters are hydrolyzed in situ by endogenous esterases, making the process traceless. This strategy provides unprecedented opportunities for the delivery of functional enzymes into human cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.