ArticleMetabolomics : Official journal of the Metabolomic Society2019
Metabolomics analysis reveals global acetoin stress response of Bacillus licheniformis.
Article in Metabolomics : Official journal of the Metabolomic Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 54 citations in OpenAlex.
- System reconstruction ofSynthetic and systems biotechnology · 2027Article
- Transcriptomic and metabolomic analysis of the antibacterial mechanism of sanguinarine against Enterobacter cloacae in vitro.BMC microbiology · 2025Article
- Systematic Adaptation of Bacillus licheniformis to 2-Phenylethanol Stress.Applied and environmental microbiology · 2023Article
- Downregulation of HbFPS1 affects rubber biosynthesis of Hevea brasiliensis suffering from tapping panel dryness.The Plant journal : for cell and molecular biology · 2023Article
- Metabolic Engineering ofMicroorganisms · 2023Article
- Article
- Sample Preparation in Microbial Metabolomics: Advances and Challenges.Advances in experimental medicine and biology · 2023Article
- Identification of Key Genes during CaApplied and environmental microbiology · 2022Article
- Mechanisms of Acetoin Toxicity and Adaptive Responses in an Acetoin-Producing Species, Lactococcus lactis.Applied and environmental microbiology · 2021Article
- Exploitation of ammonia-inducible promoters for enzyme overexpression in Bacillus licheniformis.Journal of industrial microbiology & biotechnology · 2021Article
- Antibacterial Activity and Mechanism of Linalool againstMolecules (Basel, Switzerland) · 2021Article
- A Green Route for High-Yield Production of Tetramethylpyrazine From Non-Food Raw Materials.Frontiers in bioengineering and biotechnology · 2021Article
- Integrative Metabolomics to Identify Molecular Signatures of Responses to Vaccines and Infections.Metabolites · 2020Review
- Article
- Engineering central pathways for industrial-level (3R)-acetoin biosynthesis in Corynebacterium glutamicum.Microbial cell factories · 2020Article
- Metabolic Engineering ofFrontiers in bioengineering and biotechnology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionAcetoin serves as a high value-added platform with a broad range of applications, and can be effectively produced by Bacillus licheniformis. However, its toxicity to the producing strain hinders the higher acetoin production, and current knowledge about the acetoin resistance mechanisms of B. licheniformis is quite limited.
objectivesTo comprehensively investigate the metabolic changes in B. licheniformis under acetoin stress.
methodsWe used gas chromatography-mass spectrometry based untargeted metabolomics approach to measure the metabolic profiles of B. licheniformis under 20, 40 and 80 g/L acetoin stress. Transcriptional analysis was conducted to verify the metabolomics results.
resultsA total of 119 metabolites were identified in our experiment. The metabolic responses of B. licheniformis to acetoin stress were as follows: (i) pentose phosphate pathway and tricarboxylic acid (TCA) cycle were negatively affected by acetoin stress. In turn, glyoxylate cycle was activated to supply malic acid. (ii) Acetoin stress induced the accumulation of serine, valine, leucine and protective osmolytes (glycine and proline). (iii) Acetoin stress induced a higher saturated fatty acid ratio, which indicated a lower fluidity of cell membrane that could inhibit the entry of acetoin into cytoplasm. (iv) Synthesis of phosphatidylserine was enhanced, and phosphatidylethanolamine content was probably increased under acetoin stress.
conclusionsThis study revealed the metabolic perturbations of B. licheniformis to acetoin stress. In response to acetoin stress, glyoxylate cycle was activated, protective osmolytes were accumulated, saturated fatty acid ratio was elevated and synthesis of phosphatidylserine was enhanced in B. licheniformis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.