ArticleMetabolomics : Official journal of the Metabolomic Society2019
The effect of isolation methods of tomato pollen on the results of metabolic profiling.
Article in Metabolomics : Official journal of the Metabolomic Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed.
- Untargeted metabolomics suggests a trade-off between phenolamides and flavonoids in the pollen of Petunia hybrida with contrasting flower colours.BMC plant biology · 2026Article
- Buzz pollination: investigations of pollen expulsion using the discrete element method.Journal of the Royal Society, Interface · 2025Article
- Study of the Pollen Grain Metabolome under Deposition of Nitrogen and Phosphorus inInternational journal of molecular sciences · 2022Article
- Metabolomics and Transcriptomics Analysis of Pollen Germination Response to Low-Temperature in Pitaya (Frontiers in plant science · 2022Article
- Dissection of complex traits of tomato in the post-genome era.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
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7 authors.
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Abstract
introductionUntargeted metabolomics is a powerful tool to detect hundreds of metabolites within a given tissue and to compare the metabolite composition of samples in a comprehensive manner. However, with regard to pollen research such comprehensive metabolomics approaches are yet not well developed. To enable isolation of pollen that is tightly enclosed within the anthers of the flower, such as immature pollen, the current pollen isolation protocols require the use of a watery solution. These protocols raise a number of concerns for their suitability in metabolomics analyses, in view of possible metabolic activities in the pollen and contamination with anther metabolites.
objectivesWe assessed the effect of different sample preparation procedures currently used for pollen isolation for their suitability to perform metabolomics of tomato pollen.
methodsPollen were isolated using different methods and the metabolic profiles were analysed by liquid chromatography-mass spectrometry (LC-MS).
resultsOur results demonstrated that pollen isolation in a watery solution led to (i) rehydration of the pollen grains, inducing marked metabolic changes in flavonoids, phenylpropanoids and amino acids and thus resulting in a metabolite profile that did not reflect the one of mature dry pollen, (ii) hydrolysis of sucrose into glucose and fructose during subsequent metabolite extraction, unless the isolated and rehydrated pollen were lyophilized prior to extraction, and (iii) contamination with anther-specific metabolites, such as alkaloids, thus compromising the metabolic purity of the pollen fraction.
conclusionWe conclude that the current practices used to isolate pollen are suboptimal for metabolomics analyses and provide recommendations on how to improve the pollen isolation protocol, in order to obtain the most reliable metabolic profile from pollen tissue.
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