ArticleMetabolomics : Official journal of the Metabolomic Society2017
Metabolomic study of the response to cold shock in a strain of Pseudomonas syringae isolated from cloud water.
Article in Metabolomics : Official journal of the Metabolomic Society, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- A look into the virosphere of clouds: A world yet to be explored.Current research in microbial sciences · 2026Article
- Bacterial osmoprotectants-a way to survive in saline conditions and potential crop allies.FEMS microbiology reviews · 2025Review
- Multi-kingdom microbial assemblage modulates its metabolism under contrasted cloud conditions.ISME communications · 2025Article
- Mechanistic insights into sulfur source-driven physiological responses and metabolic reorganization in the fuel-biodesulfurizingApplied and environmental microbiology · 2023Article
- Metabolomics and modelling approaches for systems metabolic engineering.Metabolic engineering communications · 2022Review
- Microbial ecology of the atmosphere.FEMS microbiology reviews · 2022Review
- Kaolinite weakens the co-stress of ampicillin and tetracycline on Escherichia coli through multiple pathways.Environmental science and pollution research international · 2021Article
- Determining the Different Mechanisms Used byFrontiers in microbiology · 2020Article
- Metabolic modulations of Pseudomonas graminis in response to HScientific reports · 2019Article
- Effect of endogenous microbiota on the molecular composition of cloud water: a study by Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS).Scientific reports · 2019Article
- Metatranscriptomic exploration of microbial functioning in clouds.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionActive microorganisms have been recently discovered in clouds, thus demonstrating the capacity of microorganisms to exist in harsh environments, including exposure to UV and oxidants, osmotic and cold shocks, etc. It is important to understand how microorganisms respond to and survive such stresses at the metabolic level.
objectivesThe objective of this work is to assess metabolome modulation in a strain of Pseudomonas syringae isolated from cloud water and facing temperature downshift from 17 to 5 °C by identifying key molecules and pathways of the response/adaptation to cold shock.
methodsBacterial extracts from suspensions of cells grown at 17 °C and further incubated in microcosms at 5 and 17 °C to mimic cloud conditions were analysed by combining LC-MS and NMR; the results were evaluated in comparison to similar suspensions kept at constant temperature. The differences in the metabolome profiles were deciphered using multivariate statistics (PLS-DA).
resultsKey cold shock biomarkers were observed, including cryoprotectants (trehalose, glucose, glycerol, carnitine, glutamate), antioxidants (glutathione and carnitine) and their precursors, alkaloids (bellendine and slaframine) and metabolites involved in energy metabolism (ATP, carbohydrates). Furthermore, new short peptides (nine dipeptides and a tetrapeptide) were found that have no known function.
conclusionsThis study shows that in response to cold temperatures, Pseudomonas syringae PDD-32b-74 demonstrates numerous metabolism modifications to counteract the impacts of low temperatures.
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