ArticleJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism2020
Two-photon microscopic imaging of capillary red blood cell flux in mouse brain reveals vulnerability of cerebral white matter to hypoperfusion.
Article in Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 62 citations in OpenAlex.
- Cortical OProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Rewiring brain structural and functional disconnection with acupuncture in rat model of vascular cognitive impairment and dementia.Biological research · 2026Article
- Diurnal differences in cerebral microvascular blood flow and oxygen delivery across brain regions in awake mice.Neurophotonics · 2026Article
- Tissue-type Differences in Focused Ultrasound and Microbubble-mediated Drug Delivery to the Brain Exist at Vessel Level.Theranostics · 2026Article
- Biophysically constrained dynamical modelling of the brain using multimodal magnetic resonance imaging.Brain research bulletin · 2025Article
- The vascular contribution to cognitive decline in ageing and dementia.Nature reviews. Neuroscience · 2025Review
- Impaired capillary-venous drainage contributes to gliosis and demyelination in mouse white matter during aging.Nature neuroscience · 2025Article
- Physiological and injury-induced microglial dynamics across the lifespan.Cell reports · 2025Article
- Depth-dependent contributions of various vascular zones to cerebral autoregulation and functional hyperemia: An in-silico analysis.PloS one · 2025Article
- Out-of-focus signal rejection forBiomedical optics express · 2025Article
- Distal activity patterns shape the spatial specificity of neurovascular coupling.Nature neuroscience · 2024Article
- Capillary regression leads to sustained local hypoperfusion by inducing constriction of upstream transitional vessels.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Non-degenerate two-photon imaging of deep rodent cortex using indocyanine green in the water absorption window.Biomedical optics express · 2024Article
- Non-Degenerate Two-Photon Imaging of Deep Rodent Cortex using Indocyanine Green in the water absorption window.bioRxiv : the preprint server for biology · 2024Article
- A brief overview of a mouse model of cerebral hypoperfusion by bilateral carotid artery stenosis.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2023Review
- Aerobic exercise reverses aging-induced depth-dependent decline in cerebral microcirculation.eLife · 2023Article
- Cortical microvascular blood flow velocity mapping by combining dynamic light scattering optical coherence tomography and two-photon microscopy.Journal of biomedical optics · 2023Article
- Differential reductions in the capillary red-blood-cell flux between retina and brain under chronic global hypoperfusion.Neurophotonics · 2023Article
- Article
- Deep Imaging to Dissect Microvascular Contributions to White Matter Degeneration in Rodent Models of Dementia.Stroke · 2023Review
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Authors and funding
14 authors at 3 institutions in 1 country.
Funding
Abstract
Despite the importance of understanding the regulation of microvascular blood flow in white matter, no data on subcortical capillary blood flow parameters are available, largely due to the lack of appropriate imaging methods. To address this knowledge gap, we employed two-photon microscopy using a far-red fluorophore Alexa680 and photon-counting detection to measure capillary red blood cell (RBC) flux in both cerebral gray and white matter, in isoflurane-anesthetized mice. We have found that in control animals, baseline capillary RBC flux in the white matter was significantly higher than in the adjacent cerebral gray matter. In response to mild hypercapnia, RBC flux in the white matter exhibited significantly smaller fractional increase than in the gray matter. Finally, during global cerebral hypoperfusion, RBC flux in the white matter was reduced significantly in comparison to the controls, while RBC flux in the gray matter was preserved. Our results suggest that blood flow in the white matter may be less efficiently regulated when challenged by physiological perturbations as compared to the gray matter. Importantly, the blood flow in the white matter may be more susceptible to hypoperfusion than in the gray matter, potentially exacerbating the white matter deterioration in brain conditions involving global cerebral hypoperfusion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.