Evidence map›Paper›PMID 30827000›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2019

Increase of Candida antarctica lipase B production under PGK promoter in Pichia pastoris: effect of multicopies.

Julia Macedo Robert, Maritza Ocampo Betancur, Antonio Carlos Oliveira Machado, Andrelisse Arruda, Viviane Castelo Branco Reis, Rodrigo Volcan Almeida, Fernando Araripe Gonçalves Torres, Pau Ferrer Alegre, Francisco Valero, Denise Maria Guimarães Freire

Open access · bronzeAbstract read
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Multicopy Expression of the Marine Antimicrobial Peptide SpgillcinProbiotics and antimicrobial proteins · 2026
    Article
  3. Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Julia Macedo RobertInstitute of Chemistry, Department of Biochemistry, Laboratory of Microbial Biotechnology, Federal University of Rio de Janeiro, Av. Athos da Silveira Ramos, 149 - CT - Bloco A - Sala 549-1 - Cidade Universitária - Ilha do Fundão, Rio de Janeiro, RJ, 21941-909, Brazil.
Maritza Ocampo BetancurDepartment of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília, DF, 70910-900, Brazil.
Antonio Carlos Oliveira MachadoInstitute of Chemistry, Department of Biochemistry, Laboratory of Microbial Biotechnology, Federal University of Rio de Janeiro, Av. Athos da Silveira Ramos, 149 - CT - Bloco A - Sala 549-1 - Cidade Universitária - Ilha do Fundão, Rio de Janeiro, RJ, 21941-909, Brazil.
Andrelisse ArrudaDepartment of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília, DF, 70910-900, Brazil.
Viviane Castelo Branco ReisDepartment of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília, DF, 70910-900, Brazil.
Rodrigo Volcan AlmeidaInstitute of Chemistry, Department of Biochemistry, Laboratory of Molecular Microbiology and Proteins, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Fernando Araripe Gonçalves TorresDepartment of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília, DF, 70910-900, Brazil.
Pau Ferrer AlegreDepartment of Chemical, Biological and Environmental Engineering, Escola d'Enginyeria, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain.
Francisco ValeroDepartment of Chemical, Biological and Environmental Engineering, Escola d'Enginyeria, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain. francisco.valero@uab.cat.
Denise Maria Guimarães FreireInstitute of Chemistry, Department of Biochemistry, Laboratory of Microbial Biotechnology, Federal University of Rio de Janeiro, Av. Athos da Silveira Ramos, 149 - CT - Bloco A - Sala 549-1 - Cidade Universitária - Ilha do Fundão, Rio de Janeiro, RJ, 21941-909, Brazil. freire@iq.ufrj.br.
Universidade de Brasília · BRUniversidade Federal do Rio de Janeiro · BRUniversitat Autònoma de Barcelona · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The effect of gene dosage on the production of Candida antarctica lipase B (CalB) in the methylotrophic yeast Komagataella phaffii, at high densities in a simple medium containing crude glycerin as the sole carbon source, is described. The use of crude glycerin, the main by-product of biodiesel production from vegetable oils, will reduce the production cost of the bioprocess. Two K. phaffii strains were constructed with one or three copies of LipB, an optimized version of the gene encoding CalB under the control of the constitutive P

Indexed as

CandidaFungal ProteinsGene DosageGlycerolLipasePichiaPromoter Regions, GeneticSaccharomycetalesFungal ProteinsGlycerolLipaselipase B, Candida antarcticaCandida antarctica, lipase BConstitutive expressionGene dosageKomagataella phaffiiMulticopy vector integration

Identifiers

PMID30827000
PMCPMC6863280
OpenAlexW2920161047

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.