ArticleScientific reports2019
High accuracy, fiducial marker-based image registration of correlative microscopy images.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- µCodes: A Universal Grid Platform for Microscale Mapping, Microscopy Navigation, and Multimodal Imaging.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Correlative light and electron microscopy imaging of proteinaceous deposits in cell cultures and brain tissues.Acta neuropathologica communications · 2025Article
- Characterization of human trabecular bone across multiple length scales using a correlative approach combining X-ray tomography with LaserFIB and plasma FIB-SEM.Scientific reports · 2024Article
- Imaging of Isolated Exosomes by Correlative Microscopy.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2024Article
- Correlative Imaging to Detect Rare HIV Reservoirs and Associated Damage in Tissues.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Bimodal endocytic probe for three-dimensional correlative light and electron microscopy.Cell reports methods · 2022Article
- Quantitative correlative microscopy reveals the ultrastructural distribution of endogenous endosomal proteins.The Journal of cell biology · 2022Article
- Correlative Organelle Microscopy: Fluorescence Guided Volume Electron Microscopy of Intracellular Processes.Frontiers in cell and developmental biology · 2022Article
- α-Synuclein fibrils subvert lysosome structure and function for the propagation of protein misfolding between cells through tunneling nanotubes.PLoS biology · 2021Article
- A Comparative Study of Automatic Localization Algorithms for Spherical Markers within 3D MRI Data.Brain sciences · 2021Article
- TEM, SEM, and STEM-based immuno-CLEM workflows offer complementary advantages.Scientific reports · 2021Article
- Fluorescent and Electron-Dense Green Color Emitting Nanodiamonds for Single-Cell Correlative Microscopy.Molecules (Basel, Switzerland) · 2020Article
- Photonic-chip assisted correlative light and electron microscopy.Communications biology · 2020Article
- Correlative light and scanning electron microscopy (CLSEM) for analysis of bacterial infection of polarized epithelial cells.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence microscopy (FM) and electron microscopy (EM) are complementary techniques. FM affords examination of large fields of view and identifying regions of interest but has a low resolution. EM exhibits excellent resolution over a limited field of view. The combination of these two techniques, correlative microscopy, received considerable interest in the past years and has proven its potential in biology and material science. Accurate correlation of FM and EM images is, however, challenging due to the differences in contrast mechanism, size of field of view and resolution. We report an accurate, fast and robust method to correlate FM and EM images using low densities of fiducial markers. Here, 120 nm diameter fiducial markers consisting of fluorescently labelled silica coated gold nanoparticles are used. The method relies on recording FM, low magnification EM and high magnification EM images. Two linear transformation matrices are constructed, FM to low magnification EM and low magnification EM to high magnification EM. Combination of these matrices results in a high accuracy transformation of FM to high magnification EM coordinates. The method was tested using two different transmission electron microscopes and different Tokuyasu and Lowicryl sections. The overall accuracy of the correlation method is high, 5-30 nm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.