ArticleScientific reports2019
Lifeact-GFP alters F-actin organization, cellular morphology and biophysical behaviour.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 50 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
50 citing papers in PubMed, 77 citations in OpenAlex.
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- MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox for use in vertebrate model systems.Development (Cambridge, England) · 2025Article
- Modulation of Estrogen Receptor Activity by the Phytoalexin Tuberosin Produced from Elicited Kudzu (Journal of natural products · 2025Article
- Nascent actin dynamics and the disruption of calcium dynamics by actin arrest in developing neural cell networks.Communications biology · 2025Article
- Characterization, Enrichment, and Computational Modeling of Cross-Linked Actin Networks in Transformed Trabecular Meshwork Cells.Investigative ophthalmology & visual science · 2025Article
- Nuclear N-WASP Induces Actin Polymerization in the Nucleus with Cortactin as an Essential Factor.Cells · 2025Article
- Nanotubules and Cellular Communication in Trabecular Meshwork Cells.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Development of label-free cell tracking for discrimination of the heterogeneous mesenchymal migration.PloS one · 2025Article
- Design and Use of AsLOV2-Based Optogenetic Tools for Actin Imaging.Methods in molecular biology (Clifton, N.J.) · 2025Article
- ARP2/3 regulates cell surface dynamics of CeTOCA-1 inJournal of biosciences · 2025Article
- Improved tools for live imaging of F-actin structures in yeast.Molecular biology of the cell · 2024Article
- Characterization, enrichment, and computational modeling of cross-linked actin networks in trabecular meshwork cells.bioRxiv : the preprint server for biology · 2024Article
- Designed Ankyrin Repeat Proteins as Actin Labels of Distinct Cytoskeletal Structures in Living Cells.ACS nano · 2024Article
- Cooperative actin filament nucleation by the Arp2/3 complex and formins maintains the homeostatic cortical array in Arabidopsis epidermal cells.The Plant cell · 2024Article
- Imaging actin organisation and dynamics in 3D.Journal of cell science · 2024Article
- Overexpression of Lifeact in theFrontiers in cell and developmental biology · 2024Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Live-imaging techniques are at the forefront of biology research to explore behaviour and function from sub-cellular to whole organism scales. These methods rely on intracellular fluorescent probes to label specific proteins, which are commonly assumed to only introduce artefacts at concentrations far-exceeding routine use. Lifeact, a small peptide with affinity for actin microfilaments has become a gold standard in live cell imaging of the cytoskeleton. Nevertheless, recent reports have raised concerns on Lifeact-associated artefacts at the molecular and whole organism level. We show here that Lifeact induces dose-response artefacts at the cellular level, impacting stress fibre dynamics and actin cytoskeleton architecture. These effects extend to the microtubule and intermediate filament networks as well as the nucleus, and ultimately lead to altered subcellular localization of YAP, reduced cell migration and abnormal mechanical properties. Our results suggest that reduced binding of cofilin to actin filaments may be the underlying cause of the observed Lifeact-induced cellular artefacts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.