Evidence map›Paper›PMID 30822687›Full record

ArticleBiosensors & bioelectronics2019

An ultra-sensitive capacitive microwire sensor for pathogen-specific serum antibody responses.

Lei Wang, Jessica E Filer, Meghan M Lorenz, Charles S Henry, David S Dandy, Brian J Geiss

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 1 pooled it
4.9field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Article
  5. Review
  6. Review
  7. Review
  8. Article
  9. Article
  10. Article
  11. Zika virus serological diagnosis: commercial tests and monoclonal antibodies as tools.The journal of venomous animals and toxins including tropical diseases · 2020
    Review
  12. Review
  13. Review-Chemical and Biological Sensors for Viral Detection.Journal of the Electrochemical Society · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Lei WangSchool of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA.
Jessica E FilerCell and Molecular Biology Program, Colorado State University, Fort Collins, CO 80523, USA; Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
Meghan M LorenzDepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
Charles S HenrySchool of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA; Department of Chemistry, Colorado State University, Fort Collins, CO 80523, USA.
David S DandySchool of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA; Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO 80523, USA. Electronic address: David.Dandy@colostate.edu.
Brian J GeissSchool of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA; Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA. Electronic address: Brian.Geiss@colostate.edu.
Colorado State University · US

Funding

Mechanism of Flavivirus RNA CappingR01AI132668 · NIAID · COLORADO STATE UNIVERSITY · PI GEISS, BRIAN · 2018 to 2022
$1.9M
A High-Throughput Screen for Antiviral Inhibitors of the Alphavirus RNA Capping EnzymeR01AI114675 · NIAID · COLORADO STATE UNIVERSITY · PI GEISS, BRIAN · 2015 to 2017
$1.1M
NIAID NIH HHS R01 AI114675NIAID NIH HHS R01 AI132668
6 · The paper itself

Abstract

Detection of viral infection is commonly performed using serological techniques like the enzyme-linked immunosorbent assay (ELISA) to detect antibody responses. Such assays may also be used to determine the infection phase based on isotype prevalence. However, ELISAs demonstrate limited sensitivity and are difficult to perform at the point of care. Here, we present a novel technique for label-free, rapid detection of ultra-low concentrations of virus specific antibodies. We have developed a simple, robust capacitive biosensor using microwires coated with Zika or Chikungunya virus envelope antigen. With little discernable nonspecific binding, the sensor can detect as few as 10 antibody molecules in a small volume (10 molecules/30 µL) within minutes. It can also be used to rapidly, specifically, and accurately determine the isotype of antigen-specific antibodies. Finally, we demonstrate that anti-Zika virus antibody can be sensitively and specifically detected in dilute mouse serum and can be isotyped using the sensor. Overall, our findings suggest that our microwire sensor platform has the potential to be used as a reliable, sensitive, and inexpensive diagnostic tool to detect immune responses at the point of care.

Indexed as

Biosensing TechniquesAntibodies, ViralAntibody FormationHumansImmunoglobulin GImmunoglobulin MViral Envelope ProteinsZika VirusZika Virus InfectionAntibodies, ViralImmunoglobulin GImmunoglobulin MViral Envelope ProteinsAntibody detectionCapacitive affinity biosensorIsotypeMicrowiresMouse serumZika virus

Identifiers

PMID30822687
PMCPMC6422737
OpenAlexW2912270675

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.