ArticleFolia microbiologica2019
Molecular detection and identification of Wolbachia endosymbiont in fleas (Insecta: Siphonaptera).
Article in Folia microbiologica, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Xenopsylla buxtoni fleas as a dominant species harboring multiple infections of Wolbachia lineages in the ancient plague epicenters of Iran.PLoS neglected tropical diseases · 2026Article
- An Earliest Endosymbiont,International journal of molecular sciences · 2020Article
- Isolation and Propagation of Laboratory Strains and a Novel Flea-Derived Field Strain ofMicroorganisms · 2020Article
- Ticks, fleas and endosymbionts in the ectoparasite fauna of the black-eared opossum Dipelphis aurita in Brazil.Experimental & applied acarology · 2020Article
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Authors and funding
7 authors.
Funding
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Abstract
The aim of this study was to determine the presence and prevalence of Wolbachia bacteria in natural population of fleas (Insecta: Siphonaptera) in Turkey, and to exhibit the molecular characterization and the phylogenetic reconstruction at the positive isolates with other species in GenBank, based on 16S rDNA sequences. One hundred twenty-four flea samples belonging to the species Ctenocephalides canis, C. felis, and Pulex irritans were collected from animal shelters in Kayseri between January and August 2017. All flea species were individually screened for the presence of Wolbachia spp. by polymerase chain reaction (PCR) targeting the 16S ribosomal RNA gene. According to PCR analyses, Wolbachia spp. were found prevalent in C. canis and P. irritans fleas, while it was not detected in the C. felis species. Totally, 20 isolates were purified from agarose gel and sequenced with the same primers for molecular characterization and phylogenetic analyses. The sequence analyses revealed 17 polymorphic sites and 2 genetically different Wolbachia isolates, representing two different haplotypes in two flea species. The distribution patterns, molecular characterization, and phylogenetic status of Wolbachia spp. of fleas in Turkey are presented for the first time with this study. Understanding of the role of Wolbachia in vector biology may provide information for developing Wolbachia-based biological control tools.
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