Evidence map›Paper›PMID 30820469›Full record

ArticleCommunications biology2019

Solid immersion microscopy images cells under cryogenic conditions with 12 nm resolution.

Lin Wang, Benji Bateman, Laura C Zanetti-Domingues, Amy N Moores, Sam Astbury, Christopher Spindloe, Michele C Darrow, Maria Romano, Sarah R Needham, Konstantinos Beis and 3 more

Abstract read
In one paragraph

Article in Communications biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Article
  2. Article
  3. Time-deterministic cryo-optical microscopy.Light, science & applications · 2025
    Article
  4. Review
  5. Recent advances in correlative cryo-light and electron microscopy.Current opinion in structural biology · 2024
    Review
  6. Review
  7. Article
  8. Review
  9. Article
  10. Review
  11. Article
  12. Review
  13. Article
  14. Article
  15. Review
  16. Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021
    Review
  17. Review
  18. Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Lin WangCentral Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, Didcot, Oxford, OX11 0QX, UK.ORCID http://orcid.org/0000-0001-9141-5217
Benji BatemanCentral Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, Didcot, Oxford, OX11 0QX, UK.
Laura C Zanetti-DominguesCentral Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, Didcot, Oxford, OX11 0QX, UK.
Amy N MooresCentral Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, Didcot, Oxford, OX11 0QX, UK.ORCID http://orcid.org/0000-0002-7254-5814
Sam AstburyCentral Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, Didcot, Oxford, OX11 0QX, UK.
Christopher SpindloeCentral Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, Didcot, Oxford, OX11 0QX, UK.
Michele C DarrowDiamond Light Source, Harwell Science and Innovation Campus, Didcot, OX11 0DE, UK.
Maria RomanoDepartment of Life Sciences, Imperial College London, London, SW7 2AZ, UK.
Sarah R NeedhamCentral Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, Didcot, Oxford, OX11 0QX, UK.
Konstantinos BeisDepartment of Life Sciences, Imperial College London, London, SW7 2AZ, UK.
Daniel J RolfeCentral Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, Didcot, Oxford, OX11 0QX, UK.
David T ClarkeCentral Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, Didcot, Oxford, OX11 0QX, UK.
Marisa L Martin-FernandezCentral Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, Didcot, Oxford, OX11 0QX, UK. marisa.martin-fernandez@stfc.ac.uk.

Funding

Medical Research Council MC_EX_MR/K015591/1Medical Research Council MR/N020103/1
6 · The paper itself

Abstract

Super-resolution fluorescence microscopy plays a crucial role in our understanding of cell structure and function by reporting cellular ultrastructure with 20-30 nm resolution. However, this resolution is insufficient to image macro-molecular machinery at work. A path to improve resolution is to image under cryogenic conditions. This substantially increases the brightness of most fluorophores and preserves native ultrastructure much better than chemical fixation. Cryogenic conditions are, however, underutilised because of the lack of compatible high numerical aperture objectives. Here, using a low-cost super-hemispherical solid immersion lens (

Indexed as

AnimalsATP-Binding Cassette TransportersCHO CellsCricetinaeCricetulusCryoelectron MicroscopyCytological TechniquesErbB ReceptorsEscherichia coliEscherichia coli ProteinsFluorescent DyesGreen Fluorescent ProteinsMaleimidesMicroscopy, FluorescenceReproducibility of ResultsAlexa Fluor 488 C5-maleimideATP-Binding Cassette TransportersErbB ReceptorsEscherichia coli ProteinsFluorescent DyesGreen Fluorescent ProteinsMaleimidesMcjD protein, E coli

Identifiers

PMID30820469
PMCPMC6385270

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.