Evidence map›Paper›PMID 30820344›Full record

ArticleFuture science OA2019

Confronting two biomolecular techniques to detect

Pieranna Chiarella, Renata Sisto, Ario de Marco

Abstract read
In one paragraph

Article in Future science OA, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Pieranna ChiarellaINAIL Research - Department of Occupational & Environmental Medicine, Epidemiology & Hygiene. Via Fontana Candida 1 - 00078 Monte Porzio Catone (RM), Italy.
Renata SistoINAIL Research - Department of Occupational & Environmental Medicine, Epidemiology & Hygiene. Via Fontana Candida 1 - 00078 Monte Porzio Catone (RM), Italy.
Ario de MarcoLaboratory for Environmental & Life Sciences - University of Nova Gorica, Vipavska 13, S-5000 Nova Gorica, Slovenia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimGene polymorphism biomarkers identify individual susceptibility to environmental and occupational hazards. The conventional approach considers polymerase chain reaction (PCR) followed by restriction fragment length polymorphism analysis (RFLP), a reliable but expensive and time-consuming two-step procedure. Therefore we evaluated the simpler method confronting two-pair primers (CTPP)-PCR for its robustness and applicability to epidemiologic studies. MATERIALS &

methodsWe compared CTPP-PCR and PCR-RFLP techniques to detect two

resultsCTPP-PCR produced contradictory results and required the orthogonal technique for confirming the data.

conclusionIn contrast to PCR-RFLP, CTPP-PCR of

Indexed as

biomarkerconfronting two-pair primersgene polymorphismNRF2oxidative stressPCRRFLPsusceptibility

Identifiers

PMID30820344
PMCPMC6391633

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.