ArticleMolecular vision2019
Comparison of histologic findings in age-related macular degeneration with RPE flatmount images.
Article in Molecular vision, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 21 citations in OpenAlex.
- Photodegradable Hydrogels for On-Demand Modeling of Age-Related Spatiotemporal ECM Deformation.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Cell loss disrupts mechanical homeostasis to drive retinal pigment epithelium ageing-like phenotype in vitro.Nature communications · 2026Article
- The intersection of mitochondria, lipids, and ferroptosis: a new avenue for dry age-related macular degeneration.Apoptosis : an international journal on programmed cell death · 2025Review
- High-Resolution Imaging and Interpretation of Three-Dimensional RPE Sheet Structure.Biomolecules · 2025Article
- Bruch's membrane heparan sulfate retains lipoproteins in the early stages of age-related macular degeneration.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Loss of Pigment Epithelium Derived Factor Sensitizes C57BL/6J Mice to Light-Induced Retinal Damage.bioRxiv : the preprint server for biology · 2024Article
- The impact of substrate stiffness on morphological, transcriptional and functional aspects in RPE.Scientific reports · 2024Article
- Epithelial mechanics are maintained by inhibiting cell fusion with age in Drosophila.Journal of cell science · 2023Article
- A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration.Journal of visualized experiments : JoVE · 2023Article
- Sub-Retinal Injection of Human Lipofuscin in the Mouse - A Model of "Dry" Age-Related Macular Degeneration?Aging and disease · 2023Article
- Fluorescence Lifetime and Spectral Characteristics of Subretinal Drusenoid Deposits and Their Predictive Value for Progression of Age-Related Macular Degeneration.Investigative ophthalmology & visual science · 2022Article
- Single-cell-resolution map of human retinal pigment epithelium helps discover subpopulations with differential disease sensitivity.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Prph2 disease mutations lead to structural and functional defects in the RPE.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Extracellular matrix dysfunction in Sorsby patient-derived retinal pigment epithelium.Experimental eye research · 2022Article
- Intravitreal fluorogold tracing as a method to label retinal neurons and the retinal pigment epithelium.Neural regeneration research · 2021Article
- Electrophysiologic and Morphologic Strain Differences in a Low-Dose NaIO3-Induced Retinal Pigment Epithelium Damage Model.Translational vision science & technology · 2021Article
- Computational Model-Based Estimation of Mouse Eyeball Structure From Two-Dimensional Flatmount Microscopy Images.Translational vision science & technology · 2021Article
- Transscleral Optical Phase Imaging of the Human Retina.Nature photonics · 2020Article
- Quantitative Fundus Autofluorescence in Rhesus Macaques in Aging and Age-Related Drusen.Investigative ophthalmology & visual science · 2020Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
Purpose: To visualize and analyze ex vivo flatmounted human RPE morphology from patients with age-related macular degeneration (AMD), and to compare the morphology with histologic findings. To establish whether the sub-RPE structures identified en face in RPE flatmount preparations are drusen with histopathological registration in serial sections. To detect characteristic patterns found en face in RPE with the same structures in histological cross sections from eyes from cadavers of patients with AMD. Methods: Twenty-eight postmortem eyes from 14 patients (16 eyes with AMD and 12 age-matched control eyes) were oriented and microdissected yielding a RPE-choroid preparation. The tissues were flatmounted, stained with Alexa Fluor 635 Phalloidin (AF635-phalloidin) for f-actin and propidium iodide for DNA, and imaged using confocal microscopy. Portions of tissue from macular regions were processed for electron microscopic examination. After confocal imaging, the samples were remounted for histologic processing, embedded in paraffin, and serially sectioned perpendicular to the plane of the RPE-choroid sheet. Scaled two-dimensional (2D) maps of drusen locations found with the histological cross sections were constructed and correlated with the en face confocal microscopic images. Results: Twenty-eight postmortem eyes with a mean time of death to tissue preservation of 23.7 h (range 8.0–51 h) from 14 donors (seven women and seven men) with an average age of 78 years (range 60–93 years) were evaluated. Eight donors had AMD, and six served as controls. Scattered small, hard drusen were present in the periphery of the eyes with AMD and the healthy eyes. The macular region of the eyes with AMD contained small (<63 µm), medium (63.0–124 µm), and large ( Conclusions: Confocal images of flatmounts from eyes with AMD showed RPE patterns overlying various types of drusen and geographic atrophy that correlated with histologic characteristics. We propose RPE repair mechanisms that may result in the patterns that we observed.
Indexed as
Identifiers
30820143PMC6377373W2914052007What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.