ArticleBMC plant biology2019
The role of protein-protein interactions mediated by the PB1 domain of NLP transcription factors in nitrate-inducible gene expression.
Article in BMC plant biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 89 citations in OpenAlex.
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- Progress and new questions in understanding the mechanisms controlling plant nitrogen responses.Plant biotechnology (Tokyo, Japan) · 2026Article
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- Identification and expression characteristics of NIN-like protein (NLP) gene family in cucumber plant (Cucumis sativus L.).BMC plant biology · 2025Article
- Nitrogen-Driven Orchestration of Lateral Root Development: Molecular Mechanisms and Systemic Integration.Biology · 2025Review
- Based on two coconut (Frontiers in plant science · 2025Article
- Genome-wide analysis of RWP-RK transcription factor family reveals its roles in nitrogen response in rice (Frontiers in plant science · 2025Article
- Carbon and nitrogen signaling regulate FLOWERING LOCUS C and impact flowering time in Arabidopsis.Plant physiology · 2024Article
- Genome-Wide Identification ofInternational journal of molecular sciences · 2024Article
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- Analysis of huanglongbing-associated RNA-seq data reveals disturbances in biological processes withinFrontiers in plant science · 2024Article
- Genomic characterization of a nematode tolerance locus in sugar beet.BMC genomics · 2023Article
- Finding Balance in Adversity: Nitrate Signaling as the Key to Plant Growth, Resilience, and Stress Response.International journal of molecular sciences · 2023Review
- Genome-Wide Survey of theInternational journal of molecular sciences · 2023Article
- Genome-Wide Identification and Expression Analysis ofInternational journal of molecular sciences · 2023Article
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- The Arabidopsis transcription factor NLP2 regulates early nitrate responses and integrates nitrate assimilation with energy and carbon skeleton supply.The Plant cell · 2023Article
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2 authors at 1 institution in 1 country.
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Abstract
backgroundNIN-LIKE PROTEIN (NLP) transcription factors are master regulators of nitrate-inducible gene expression in higher plants. NLP transcription factors contain a nitrate signal-responsive domain in the amino-terminal region, an RWP-RK-type DNA-binding domain in the middle, and a Phox and Bem1 (PB1) domain at the carboxy terminus. Although the PB1 domain of NLP transcription factors appears to mediate protein-protein interactions associated with nitrate-inducible gene expression in higher plants, its precise role in nitrate-inducible gene expression has not previously been characterized.
resultsYeast two-hybrid assays with the PB1 domain of the Arabidopsis transcription factor NLP7 revealed NLP-NLP interactions that required the core amino acid residues (K867, D909, D911, and E913) within the PB1 domain. Consistent with previous speculation on redundant and overlapping functions between different Arabidopsis NLP transcription factors, NLP-NLP interactions were observed between a variety of combinations of different NLP transcription factors. Furthermore, a mutated form of NLP7 that harbored amino acid substitutions at K867, D909, D911, and E913 required a far higher level of expression than wild-type NLP7 to restore nitrate-responsive gene expression and growth of nlp6 nlp7-1 double mutants. Surprisingly, however, the ability to transactivate nitrate-responsive promoters in protoplast transient expression assays was similar between wild-type and mutant forms of NLP7, suggesting that the PB1 domain was not required for transcription from naked DNA.
conclusionsProtein-protein interactions mediated by the PB1 domain of NLP transcription factors are necessary for full induction of nitrate-dependent expression of target genes in planta. The PB1 domains of NLP transcription factors may act on gene expression from chromosomal DNA via homo- and hetero-oligomerization in the presence of nitrate.
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