Evidence map›Paper›PMID 30818168›Full record

ArticleDNA repair2019

Cooperation between non-essential DNA polymerases contributes to genome stability in Saccharomyces cerevisiae.

Damon Meyer, Becky Xu Hua Fu, Monique Chavez, Sophie Loeillet, Paula G Cerqueira, Alain Nicolas, Wolf-Dietrich Heyer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in DNA repair, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Damon MeyerDepartment of Microbiology and Molecular Genetics, University of California, Davis, One Shields Avenue, Davis, CA 95616-8665, USA.
Becky Xu Hua FuDepartment of Microbiology and Molecular Genetics, University of California, Davis, One Shields Avenue, Davis, CA 95616-8665, USA.
Monique ChavezDepartment of Microbiology and Molecular Genetics, University of California, Davis, One Shields Avenue, Davis, CA 95616-8665, USA.
Sophie LoeilletInstitut Curie, PSL Research University, CNRS UMR3244, Recombination and Genetic Instability, 75248 Paris Cedex 05, France; Sorbonne Universités, UPMC University Paris 06, CNRS UMR3244, 75248 Paris Cedex 05, France.
Paula G CerqueiraDepartment of Microbiology and Molecular Genetics, University of California, Davis, One Shields Avenue, Davis, CA 95616-8665, USA.
Alain NicolasInstitut Curie, PSL Research University, CNRS UMR3244, Recombination and Genetic Instability, 75248 Paris Cedex 05, France; Sorbonne Universités, UPMC University Paris 06, CNRS UMR3244, 75248 Paris Cedex 05, France.
Wolf-Dietrich HeyerDepartment of Microbiology and Molecular Genetics, University of California, Davis, One Shields Avenue, Davis, CA 95616-8665, USA; Department of Molecular and Cellular Biology, University of California, Davis, One Shields Avenue, Davis, CA 95616-8665, USA. Electronic address: wdheyer@ucdavis.edu.
University of California, Davis · USCentre National de la Recherche Scientifique · FR

Funding

Staff InvestigatorsP30CA093373 · NCI · UNIVERSITY OF CALIFORNIA DAVIS · PI Gina M Dayton · 2002 to 2026
$84.9M
RECOMBINATIONAL MECHANISMS OF DNA REPAIR IN EUKARYOTESR01GM058015 · NIGMS · UNIVERSITY OF CALIFORNIA DAVIS · PI HEYER, WOLF-DIETRICH · 2000 to 2025
$8.8M
Training Program in Oncogenic Signals and Chromosome BiologyT32CA108459 · NCI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI HEYER, WOLF-DIETRICH · 2006 to 2020
$3.6M
UC Davis MARC Scholar ProgramT34GM083894 · NIGMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI GOMES, ALDRIN V. · 2009 to 2019
$2.1M
NCI NIH HHS P30 CA093373NCI NIH HHS T32 CA108459NIGMS NIH HHS R01 GM058015NIGMS NIH HHS T34 GM083894
6 · The paper itself

Abstract

DNA polymerases influence genome stability through their involvement in DNA replication, response to DNA damage, and DNA repair processes. Saccharomyces cerevisiae possess four non-essential DNA polymerases, Pol λ, Pol η, Pol ζ, and Rev1, which have varying roles in genome stability. In order to assess the contribution of the non-essential DNA polymerases in genome stability, we analyzed the pol4Δ rev1Δ rev3Δ rad30Δ quadruple mutant in microhomology mediated repair, due to recent studies linking some of these DNA polymerases to this repair pathway. Our results suggest that the length and quality of microhomology influence both the overall efficiency of repair and the involvement of DNA polymerases. Furthermore, the non-essential DNA polymerases demonstrate overlapping and redundant functions when repairing double-strand breaks using short microhomologies containing mismatches. Then, we examined genome-wide mutation accumulation in the pol4Δ rev1Δ rev3Δ rad30Δ quadruple mutant compared to wild type cells. We found a significant decrease in the overall rate of mutation accumulation in the quadruple mutant cells compared to wildtype, but an increase in frameshift mutations and a shift towards transversion base-substitution with a preference for G:C to T:A or C:G. Thus, the non-essential DNA polymerases have an impact on the nature of the mutational spectrum. The sequence and functional homology shared between human and S. cerevisiae non-essential DNA polymerases suggest these DNA polymerases may have a similar role in human cells.

Indexed as

Genomic InstabilityDNA-Directed DNA PolymeraseDNA RepairMutationSaccharomyces cerevisiaeDNA-Directed DNA PolymeraseDNA polymerasesDNA repairGenome stabilityMutation accumulationYeast

Identifiers

PMID30818168
PMCPMC6443415
OpenAlexW2913118722

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.