ArticlePLoS neglected tropical diseases2019
Fast diagnosis of sporotrichosis caused by Sporothrix globosa, Sporothrix schenckii, and Sporothrix brasiliensis based on multiplex real-time PCR.
Article in PLoS neglected tropical diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 2 of them syntheses that pooled it.
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Who cites it
25 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- The Historical Burden of Sporotrichosis in Brazil: a Systematic Review of Cases Reported from 1907 to 2020.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Pooled it
- Sporotrichosis in Mexico.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Pooled it
- Veterinary application of LAMP: a simple and visual detection tool for feline sporotrichosis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Detection of Sporothrix brasiliensis in Human Tissue by Direct PCR Using Species-Specific Primers and NESTED PCR in a New Epidemic Area of Sporotrichosis.Mycopathologia · 2025Article
- Clinical and Molecular Characterization of Feline Sporotrichosis in the Brazilian Amazon: PCR-Based Identification ofAnimals : an open access journal from MDPI · 2025Article
- A novel approach for feline sporotrichosis pathogen detection based on loop-mediated isothermal amplification.Veterinary dermatology · 2025Article
- Dermatologic fungal neglected tropical diseases-Part I. Epidemiology and clinical features.Journal of the American Academy of Dermatology · 2025Review
- Extracutaneous sporotrichosis.Clinical microbiology reviews · 2025Review
- Diagnosis of Human Endemic Mycoses Caused by Thermally Dimorphic Fungi: From Classical to Molecular Methods.Journal of fungi (Basel, Switzerland) · 2024Review
- Development and evaluation of a rapid diagnostic method for Sporothrix globosa in Asia using quantitative real-time PCR.BMC infectious diseases · 2024Article
- Current situation of sporotrichosis in China.Future microbiology · 2024Review
- Rapid Molecular Diagnosis of Sporotrichosis Directly from Biological Samples from a Reference Center in Brazil.Journal of fungi (Basel, Switzerland) · 2024Article
- Case Report: Ocular Sporotrichosis-A Seven-Case Series.The American journal of tropical medicine and hygiene · 2023Article
- Evaluation of Five Non-Culture-Based Methods for the Diagnosis of Meningeal Sporotrichosis.Journal of fungi (Basel, Switzerland) · 2023Article
- Trends in Molecular Diagnostics and Genotyping Tools Applied for EmergingJournal of fungi (Basel, Switzerland) · 2022Review
- Current Progress on Epidemiology, Diagnosis, and Treatment of Sporotrichosis and Their Future Trends.Journal of fungi (Basel, Switzerland) · 2022Review
- Molecular Diagnosis of Two Major Implantation Mycoses: Chromoblastomycosis and Sporotrichosis.Journal of fungi (Basel, Switzerland) · 2022Review
- Old and New Insights intoPathogens (Basel, Switzerland) · 2022Review
- In vitro activity of Schinus terebinthifolius extract and fractions against Sporothrix brasiliensis.Memorias do Instituto Oswaldo Cruz · 2022Article
- Clinical and epidemiological aspects of feline sporotrichosis caused by Sporothrix brasiliensis and in vitro antifungal susceptibility.Veterinary research communications · 2021Article
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5 authors.
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Abstract
The accurate diagnosis of sporotrichosis and identification at the species level are critical for public health and appropriate patient management. Compared with morphological identification methods, molecular diagnostic tests are rapid and have high sensitivity and standardized operating processes. Therefore, we designed a novel multiplex real-time polymerase chain reaction (PCR) method based on the calmodulin (CAL) gene for the identification of clinically relevant Sporothrix species: S. globosa, S. schenckii s. str., and S. brasiliensis. We evaluated the assay with clinical and spiked samples and assessed its diagnostic performance by comparing the results to those of culture and species-specific PCR. Thirty-three DNA templates were used to detect assay specificity, and three plasmids were constructed to create a standard curve and determine the limits of detection (LODs). For nucleic acid detection, the sensitivity and specificity reached 100%. The LODs were 10 copies, 10 copies and 100 copies for S. globosa, S. schenckii s. str and S. brasiliensis, respectively. For the clinical samples, the positive detection rates by culture, species-specific PCR and the multiplex real-time PCR assay were 87.9% (29/33), 39.4% (13/33), and 93.9% (31/33), respectively. For the spiked samples, the positive detection rates were both 100% for S. schenckii s. str and S. brasiliensis. Based on the above results, compared with culture and other molecular diagnosis methods, the novel multiplex real-time PCR assay is effective, fast, accurate, and highly sensitive. It has a lower reaction cost and lower sample volume requirements, can detect co-infections, and allows for standardized operation and easier interpretation of results. In the future, this assay could be developed into a commercial kit for the diagnosis and identification of S. globosa, S. schenckii s. str, and S. brasiliensis.
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