ArticleAnalytical chemistry2019
Sequential Digestion with Trypsin and Elastase in Cross-Linking Mass Spectrometry.
Article in Analytical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 37 citations in OpenAlex.
- Epitope and Paratope Mapping of a SUMO-Remnant Antibody Using Cross-Linking Mass Spectrometry and Molecular Docking.Journal of proteome research · 2025Article
- High-Performance Workflow for Identifying Site-Specific Crosslinks Originating from a Genetically Incorporated, Photoreactive Amino Acid.Journal of proteome research · 2024Article
- Wheat-Based Glues in Conservation and Cultural Heritage: (Dis)solving the Proteome of Flour and Starch Pastes and Their Adhering Properties.Journal of proteome research · 2024Article
- Higher-Order Structural Organization of the Mitochondrial Proteome Charted by In Situ Cross-Linking Mass Spectrometry.Molecular & cellular proteomics : MCP · 2023Article
- Protein fibers with self-recoverable mechanical properties via dynamic imine chemistry.Nature communications · 2023Article
- Cross-linking mass spectrometry discovers, evaluates, and corroborates structures and protein-protein interactions in the human cell.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Porphyromonas gingivalis induction of TLR2 association with Vinculin enables PI3K activation and immune evasion.PLoS pathogens · 2023Article
- Biodegradation of Pine Processionary Caterpillar Silk Is Mediated by Elastase- and Subtilisin-like Proteases.International journal of molecular sciences · 2022Article
- Immobilization Systems of Antimicrobial Peptide Ib-M1 in Polymeric Nanoparticles Based on Alginate and Chitosan.Polymers · 2022Article
- Exploring the active core of a novel antimicrobial peptide, palustrin-2LTb, from the Kuatun frog,Computational and structural biotechnology journal · 2022Article
- SPACEPro: A Software Tool for Analysis of Protein Sample Cleavage for Tandem Mass Spectrometry.Journal of proteome research · 2021Article
- Selective cross-linking of coinciding protein assemblies by in-gel cross-linking mass spectrometry.The EMBO journal · 2021Article
- Cleavable Cross-Linkers and Mass Spectrometry for the Ultimate Task of Profiling Protein-Protein Interaction NetworksJournal of proteome research · 2021Review
- Comparative Analysis of the Extracellular Matrix Proteome across the Myotendinous Junction.Journal of proteome research · 2020Article
- Proteomics Using Protease Alternatives to Trypsin Benefits from Sequential Digestion with Trypsin.Analytical chemistry · 2020Article
- In Situ Structural Restraints from Cross-Linking Mass Spectrometry in Human Mitochondria.Journal of proteome research · 2020Article
- Optimized Cross-Linking Mass Spectrometry for in Situ Interaction Proteomics.Journal of proteome research · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Cross-linking mass spectrometry has become an important approach for studying protein structures and protein-protein interactions. The amino acid compositions of some protein regions impede the detection of cross-linked residues, although it would yield invaluable information for protein modeling. Here, we report on a sequential-digestion strategy with trypsin and elastase to penetrate regions with a low density of trypsin-cleavage sites. We exploited intrinsic substrate-recognition properties of elastase to specifically target larger tryptic peptides. Our application of this protocol to the TAF4-12 complex allowed us to identify cross-links in previously inaccessible regions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.