ArticleClinical implant dentistry and related research2019
TGF-β activity in acid bone lysate adsorbs to titanium surface.
Article in Clinical implant dentistry and related research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 10 citations in OpenAlex.
- Does Injectable Platelet-Rich Fibrin Combined With Autogenous Demineralized Dentine Enhance Alveolar Ridge Preservation? A Randomized Controlled Trial.Clinical oral implants research · 2025Trial
- PRMT1-SFPQ regulates intron retention to control matrix gene expression during craniofacial development.eLife · 2026Article
- Bone Allograft Acid Lysates Change the Genetic Signature of Gingival Fibroblasts.International journal of molecular sciences · 2023Article
- Bioactivation of 3D Cell-Imprinted Polydimethylsiloxane Surfaces by Bone Protein Nanocoating for Bone Tissue Engineering.ACS omega · 2022Article
- Proteomic and genomic analysis of acid dentin lysate with focus on TGF-β signaling.Scientific reports · 2021Article
- Acid bone lysates reduce bone regeneration in rat calvaria defects.Journal of biomedical materials research. Part A · 2021Article
- Cleaning Teeth Reduces the Inflammatory Response of Macrophages to Acid Dentine Lysate.International journal of molecular sciences · 2020Article
- TGFβ activity released from platelet-rich fibrin adsorbs to titanium surface and collagen membranes.Scientific reports · 2020Article
- TGF-β activity in acid bone lysate adsorbs to titanium surface.Clinical implant dentistry and related research · 2019Article
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Authors and funding
7 authors at 4 institutions in 4 countries.
Funding
Abstract
objectivesOsteoblasts lay down new bone on implant surfaces. The underlying cellular mechanism and the spatio-temporal mode of action, however, remain unclear. It can be proposed that growth factors released upon acidification by osteoclasts adsorb to the implant surface and control the early stages of osseointegration.
methodsTo simulate bone lysis by osteoclasts, titanium discs were exposed to acid bone lysate (ABL) followed by vigorous washing and seeding of oral fibroblasts. The expression of TGF-β target genes interleukin 11 (IL11) and NADPH oxidase 4 (NOX4) was evaluated by reverse transcriptase polymerase chain reaction and IL11 ELISA. TGF-β signaling activation was assessed via Smad2/3 immunofluorescence. The impact of ABL on osteogenic differentiation was determined with murine ST2 mesenchymal stromal cells.
resultsWe report here that ABL-conditioned titanium discs, independent of turned or rough surface, increased the expression of IL11 and NOX4. This increase was blocked by the TGF-β receptor 1 antagonist SB431542. Further support for the TGF-β signaling activation came from the translocation of Smad2/3 into the nucleus of oral fibroblasts. Moreover, titanium discs exposed to ABL decreased alkaline phosphatase and osteopontin in ST2 cells.
conclusionsThese in vitro findings suggest that titanium can adsorb TGF-β from ABLs. The data provide a strong impetus for studies on the protein adsorption on implant surfaces in vitro and in vivo, specifically for growth factors including bone-derived TGF-β during successful and failed osseointegration.
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