Evidence map›Paper›PMID 30816668›Full record

ArticleCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology2019

An Essential and Synergistic Role of Purinergic Signaling in Guided Migration of Corneal Epithelial Cells in Physiological Electric Fields.

Ken-Ichi Nakajima, Makiko Tatsumi, Min Zhao

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.5field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
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  7. The Dynamics of the Skin's Immune System.International journal of molecular sciences · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 2 countries.

Ken-Ichi NakajimaDepartment of Dermatology, University of California at Davis, Sacramento, CA, USA, kennakajima@ucdavis.edu.
Makiko TatsumiDepartment of Dermatology, University of California at Davis, Sacramento, CA, USA.
Min ZhaoDepartment of Dermatology, University of California at Davis, Sacramento, CA, USA.
University of California, Davis · US

Funding

Molecular Generators at Corneal Wounds Produce and regulate the Wound Electrical SignalsR01EY019101 · NEI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI ZHAO, MIN · 2010 to 2023
$5.2M
Complement Inhibitors for Macular DegenerationR44EY019191 · NEI · NOVELMED THERAPEUTICS, INC. · PI BANSAL, REKHA · 2012 to 2014
$1.3M
Complement Inhibitors for Macular DegenerationR43EY019191 · NEI · NOVELMED THERAPEUTICS, INC. · PI BANSAL, REKHA · 2009 to 2009
$244k
Air Force Office of Scientific Research - Multidisciplinary Research Program of the University Research Initiative FA9550-16-1-0052NEI NIH HHS R01 EY019101NEI NIH HHS R43 EY019191NEI NIH HHS R44 EY019191NIH HHS EY019191Research to Prevent Blindness Inc.University of California at Davis Bridging fundUniversity of California at Davis Interdepartmental seed grant
6 · The paper itself

Abstract

BACKGROUND/

aimsDirectional migration of corneal epithelial cells is essential for healing of corneal wounds, which is a robust response mediated by biochemical and bioelectrical cues. Naturally occurring electric fields at corneal wounds provide a powerful guidance cue for directional cell migration, as does extracellular ATP. Our recent large-scale siRNA library screening identified a role for purinergic signaling in the electric field-guided migration (galvanotaxis/electrotaxis) of human corneal epithelial (hTCEpi) cells.

methodsWe examined the effect of extracellular ATP on galvanotaxis of hTCEpi cells. Galvanotactic cell migration was recorded by video microscopy, and directedness and migration speed was calculated. The role of purinergic receptors in galvanotaxis regulation was evaluated by pharmacological inhibition or knocking down of P2X and P2Y receptors.

resultsAddition of ATP enhanced galvanotaxis, and most remarkably sensitized galvanotaxis response to very low level of electric fields in the physiological range (10-30 mV/mm). The stimulatory effect of extracellular ATP was diminished by apyrase treatment. Importantly, cells stimulated with extracellular ATP migrated with significantly increased directedness and speed, which were diminished by knocking down or pharmacological inhibition of P2X and P2Y receptors. Inhibition of pannexin-1 (ATP permeable channel) significantly impaired galvanotaxis. Moreover, pharmacological inhibition of ectoATPase enhanced galvanotaxis.

conclusionExtracellular ATP and physiological electric fields synergistically enhanced the galvanotaxis response of hTCEpi cells. hTCEpi cells are likely to secrete ATP actively, and purinergic signaling is down-regulated by ecto-ATPases. Both P2X and P2Y receptors coordinately play a role for galvanotaxis of hTCEpi cells.

Indexed as

Cell MovementElectromagnetic FieldsSignal TransductionAdenosine TriphosphateCell Line, TransformedEpithelial CellsEpithelium, CornealHumansReceptors, Purinergic P2XReceptors, Purinergic P2YAdenosine TriphosphateReceptors, Purinergic P2XReceptors, Purinergic P2YATPCorneal epithelial cellElectric fieldGalvanotaxisPurinergic signaling

Identifiers

PMID30816668
PMCPMC8917963
OpenAlexW2924283831

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.