ArticleScientific reports2019
Brain-stiffness-mimicking tilapia collagen gel promotes the induction of dorsal cortical neurons from human pluripotent stem cells.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
- Review
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- Leveraging Blood Components for 3D Printing Applications Through Programmable Ink Engineering Approaches.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Effects of amyloid-β-mimicking peptide hydrogel matrix on neuronal progenitor cell phenotype.Acta biomaterialia · 2024Article
- Changes in the Biomechanical Properties of Corneal Stromal Lens after Collagen Crosslinking Induced by EDC-NHS.Journal of ophthalmology · 2024Article
- Acellular fish skin for wound healing.International wound journal · 2023Review
- Practical guide for preparation, computational reconstruction and analysis of 3D human neuronal networks in control and ischaemic conditions.Development (Cambridge, England) · 2022Article
- Direct visualization of the transition status during neural differentiation by dual-fluorescent reporter human pluripotent stem cells.Stem cell reports · 2022Article
- 3D Bioprinted Neural-Like Tissue as a Platform to Study Neurotropism of Mouse-Adapted SARS-CoV-2.Advanced biology · 2022Article
- Biomaterials to enhance stem cell transplantation.Cell stem cell · 2022Review
- Biomaterials reinforced MSCs transplantation for spinal cord injury repair.Asian journal of pharmaceutical sciences · 2022Review
- Impact of hydrogel stiffness on the induced neural stem cells modulation.Annals of translational medicine · 2021Article
- Stiff-to-Soft Transition from Glass to 3D Hydrogel Substrates in Neuronal Cell Culture.Micromachines · 2021Article
- Marine-Derived Collagen as Biomaterials for Human Health.Frontiers in nutrition · 2021Review
- Light Cross-Linkable Marine Collagen for Coaxial Printing of a 3D Model of Neuromuscular Junction Formation.Biomedicines · 2020Article
- Mechanical Regulation of Nuclear Translocation in Migratory Neurons.Frontiers in cell and developmental biology · 2020Review
- How to reprogram human fibroblasts to neurons.Cell & bioscience · 2020Review
- Application of marine collagen for stem-cell-based therapy and tissue regeneration (Review).Medicine internationalReview
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanical properties of the extracellular microenvironment, including its stiffness, play a crucial role in stem cell fate determination. Although previous studies have demonstrated that the developing brain exhibits spatiotemporal diversity in stiffness, it remains unclear how stiffness regulates stem cell fate towards specific neural lineages. Here, we established a culture substrate that reproduces the stiffness of brain tissue using tilapia collagen for in vitro reconstitution assays. By adding crosslinkers, we obtained gels that are similar in stiffness to living brain tissue (150-1500 Pa). We further examined the capability of the gels serving as a substrate for stem cell culture and the effect of stiffness on neural lineage differentiation using human iPS cells. Surprisingly, exposure to gels with a stiffness of approximately 1500 Pa during the early period of neural induction promoted the production of dorsal cortical neurons. These findings suggest that brain-stiffness-mimicking gel has the potential to determine the terminal neural subtype. Taken together, the crosslinked tilapia collagen gel is expected to be useful in various reconstitution assays that can be used to explore the role of stiffness in neurogenesis and neural functions. The enhanced production of dorsal cortical neurons may also provide considerable advantages for neural regenerative applications.
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