Evidence map›Paper›PMID 30815897›Full record

ArticleEquine veterinary journal2019

The use of equine chondrogenic-induced mesenchymal stem cells as a treatment for osteoarthritis: A randomised, double-blinded, placebo-controlled proof-of-concept study.

S Y Broeckx, A M Martens, A L Bertone, L Van Brantegem, L Duchateau, L Van Hecke, M Dumoulin, M Oosterlinck, K Chiers, H Hussein and 2 more

Open access · hybridAbstract readRandomized Controlled Trial, Veterinary
PubMed Full text at PMC Publisher
In one paragraph

Article in Equine veterinary journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed, 2 pooled it
9.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 2 syntheses or guidelines pooled it, 49 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 3 countries.

S Y BroeckxGlobal Stem Cell Technology NV, Anacura Group, Evergem, Belgium.
A M MartensDepartment of Surgery and Anaesthesiology of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
A L BertoneDepartment of Veterinary Clinical Sciences, Ohio State University, Columbus, USA.
L Van BrantegemDepartment of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
L DuchateauBiometrics Research Group, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
L Van HeckeGlobal Stem Cell Technology NV, Anacura Group, Evergem, Belgium.ORCID https://orcid.org/0000-0002-8393-3855
M DumoulinDepartment of Surgery and Anaesthesiology of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
M OosterlinckDepartment of Surgery and Anaesthesiology of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.ORCID https://orcid.org/0000-0001-6486-2505
K ChiersDepartment of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
H HusseinDepartment of Veterinary Clinical Sciences, Ohio State University, Columbus, USA.
F PilleDepartment of Surgery and Anaesthesiology of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
J H SpaasGlobal Stem Cell Technology NV, Anacura Group, Evergem, Belgium.
Ghent University · BEStem Cell Technology (Taiwan) · TWThe Ohio State University · US

Funding

Global Stem cell Technology NV 130543
6 · The paper itself

Abstract

backgroundThere is a need to improve therapies for osteoarthritis in horses.

objectivesTo assess the efficacy of equine allogeneic chondrogenic-induced mesenchymal stem cells combined with equine allogeneic plasma as a novel therapy for osteoarthritis in horses. STUDY

designRandomised, double-blinded, placebo-controlled experiment.

methodsIn 12 healthy horses, osteoarthritis was induced in the metacarpophalangeal joint using an osteochondral fragment-groove model. Five weeks after surgery, horses were randomly assigned to either an intra-articular injection with chondrogenic-induced mesenchymal stem cells + equine allogeneic plasma (= intervention) or with 0.9% saline solution (= control). From surgery until the study end, horses underwent a weekly joint and lameness assessment. Synovial fluid was collected for cytology and biomarker analysis before surgery and at Weeks 5, 5 + 1d, 7, 9 and 11. At Week 11, horses were subjected to euthanasia, and the metacarpophalangeal joints were evaluated macroscopically and histologically.

resultsNo serious adverse events or suspected adverse drug reactions occurred during the study. A significant improvement in visual and objective lameness was seen with the intervention compared with the control. Synovial fluid displayed a significantly higher viscosity and a significantly lower glycosaminoglycan concentration in the intervention group. Other biomarkers or cytology parameters were not significantly different between the treatment groups. Significantly less wear lines and synovial hyperaemia were present in the intervention group. The amount of cartilage oligomeric matrix protein, collagen type II and glycosaminoglycans were significantly higher in the articular cartilage of the intervention group. MAIN LIMITATIONS: This study assessed the short-term effect of the intervention on a limited number of horses, using an osteoarthritis model. This study also included multiple statistical tests, increasing the risk of type 1 error.

conclusionsEquine allogeneic chondrogenic-induced mesenchymal stem cells combined with equine allogeneic plasma may be a promising treatment for osteoarthritis in horses. The Summary is available in Spanish - see Supporting Information.

Indexed as

ChondrogenesisAnimalsDouble-Blind MethodFemaleHorse DiseasesHorsesMaleMesenchymal Stem CellsMesenchymal Stem Cell TransplantationOsteoarthritisProof of Concept Studyallogeneichorsemetacarpophalangeal jointmodelperipheral blood

Identifiers

PMID30815897
PMCPMC6850029
OpenAlexW2918467571

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.