ArticleFEBS letters2019
Improved recombinant expression and purification of functional plant Rubisco.
Article in FEBS letters, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 46 citations in OpenAlex.
- Machine learning-assisted directed evolution of plant Rubisco.Science advances · 2026Article
- Rubisco functional integrity requires a dedicated three-step maturation pathway.Science advances · 2026Article
- The fitness landscape of a form II rubisco in a photosynthetic bacterium guides engineering of oxygen tolerance.Science advances · 2026Article
- Causes and consequences of cytonuclear incompatibility in hybrids of flowering plants.Journal of experimental botany · 2026Review
- Rubisco kinetic acclimation at the holoenzyme level.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Replacement of large subunit N terminus enabled biogenesis of different plant Rubiscos in E. coli.Plant biotechnology journal · 2025Article
- A SynBio explosion: a whole new world for Rubisco engineering.Journal of experimental botany · 2025Article
- The small subunit of Rubisco and its potential as an engineering target.Journal of experimental botany · 2023Review
- Cell-free expression of RuBisCO for ATP production in the synthetic cells.Synthetic biology (Oxford, England) · 2023Article
- A temporal gradient of cytonuclear coordination of chaperonins and chaperones during RuBisCo biogenesis in allopolyploid plants.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Discovery of a readily heterologously expressed Rubisco from the deep sea with potential for COBioresources and bioprocessing · 2021Article
- Enhancing crop yield by using Rubisco activase to improve photosynthesis under elevated temperatures.Stress biology · 2021Review
- Rubisco activase requires residues in the large subunit N terminus to remodel inhibited plant Rubisco.The Journal of biological chemistry · 2020Article
- Small subunits can determine enzyme kinetics of tobacco Rubisco expressed in Escherichia coli.Nature plants · 2020Article
- Modifying Plant Photosynthesis and Growth via Simultaneous Chloroplast Transformation of Rubisco Large and Small Subunits.The Plant cell · 2020Article
- A wish list for synthetic biology in photosynthesis research.Journal of experimental botany · 2020Article
- Elucidation and genetic intervention of COJournal of biosciences · 2020Article
- Directed Evolution of an Improved Rubisco; In Vitro Analyses to Decipher Fact from Fiction.International journal of molecular sciences · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Improving the performance of the key photosynthetic enzyme Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) by protein engineering is a critical strategy for increasing crop yields. The extensive chaperone requirement of plant Rubisco for folding and assembly has long been an impediment to this goal. Production of plant Rubisco in Escherichia coli requires the coexpression of the chloroplast chaperonin and four assembly factors. Here, we demonstrate that simultaneous expression of Rubisco and chaperones from a T7 promotor produces high levels of functional enzyme. Expressing the small subunit of Rubisco with a C-terminal hexahistidine-tag further improved assembly, resulting in a ~ 12-fold higher yield than the previously published procedure. The expression system described here provides a platform for the efficient production and engineering of plant Rubisco.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.