ArticleLab on a chip2019
A diffusion-based microfluidic device for single-cell RNA-seq.
Article in Lab on a chip, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Integration of hyperspectral imaging and transcriptomics from individual cells with SpectralSeq.Genome research · 2025Article
- Microfluidic Biochips for Single-Cell Isolation and Single-Cell Analysis of Multiomics and Exosomes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Single-cell transcriptomics in human skin research: available technologies, technical considerations and disease applications.Experimental dermatology · 2022Review
- Advancement of Single-Cell Sequencing in Medulloblastoma.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Application and prospects of single cell sequencing in tumors.Biomarker research · 2021Review
- Chemical Analysis of Single Cells and Organelles.Analytical chemistry · 2021Review
- Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics.Microfluidics and nanofluidics · 2021Review
- Review
- Microfluidic Platform for Next-Generation Sequencing Library Preparation with Low-Input Samples.Analytical chemistry · 2020Article
- Whole genome amplification of single epithelial cells dissociated from snap-frozen tissue samples in microfluidic platform.Biomicrofluidics · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Microfluidic devices provide a low-input and efficient platform for single-cell RNA-seq (scRNA-seq). Existing microfluidic devices have a complicated multi-chambered structure for handling the multi-step process involved in RNA-seq and dilution between steps is used to negate the inhibitory effects among reagents. This makes the device difficult to fabricate and operate. Here we present microfluidic diffusion-based RNA-seq (MID-RNA-seq) for conducting scRNA-seq with a diffusion-based reagent swapping scheme. This device incorporates cell trapping, lysis, reverse transcription and PCR amplification all in one simple microfluidic device. MID-RNA-seq provides high data quality that is comparable to existing scRNA-seq methods while implementing a simple device design that permits multiplexing. The robustness and scalability of the MID-RNA-seq device will be important for transcriptomic studies of scarce cell samples.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.