ArticleTissue engineering and regenerative medicine2019
A Comparative Study of the Effects of Different Decellularization Methods and Genipin-Cross-Linking on the Properties of Tracheal Matrices.
Article in Tissue engineering and regenerative medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- MSCs-Derived Decellularised Matrix: Cellular Responses and Regenerative Dentistry.International dental journal · 2024Review
- Dynamic flow for efficient partial decellularization of tracheal grafts: A preliminary rabbit study.Laryngoscope investigative otolaryngology · 2024Article
- Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine.Biomaterials research · 2023Review
- A defined road to tracheal reconstruction: laser structuring and cell support for rapid clinic translation.Stem cell research & therapy · 2022Article
- Combined Mesenchymal Stem Cells and Cartilage Acellular Matrix Injection Therapy for Osteoarthritis in Goats.Tissue engineering and regenerative medicine · 2022Article
- Hydrogel modification of 3D printing hybrid tracheal scaffold to construct an orthotopic transplantation.American journal of translational research · 2022Article
- Sterilization and disinfection methods for decellularized matrix materials: Review, consideration and proposal.Bioactive materials · 2021Review
- Rapid Preparation Method for Preparing Tracheal Decellularized Scaffolds: Vacuum Assistance and Optimization of DNase I.ACS omega · 2021Article
- Human nasal septal chondrocytes (NSCs) preconditioned on NSC-derived matrix improve their chondrogenic potential.Biomaterials research · 2021Article
- Histological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral Nerves.International journal of molecular sciences · 2021Article
- Inhibitory Effect of Topical Cartilage Acellular Matrix Suspension Treatment on Neovascularization in a Rabbit Corneal Model.Tissue engineering and regenerative medicine · 2020Article
- Tissue engineering applications in otolaryngology-The state of translation.Laryngoscope investigative otolaryngology · 2020Review
- Building Scaffolds for Tubular Tissue Engineering.Frontiers in bioengineering and biotechnology · 2020Review
- Post-decellularization techniques ameliorate cartilage decellularization process for tissue engineering applications.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDifferent decellularization methods can affect the integrity and the biomechanical and biocompatible properties of the tracheal matrix. Natural cross-linking with genipin can be applied to improve those properties. The goals of this study were to evaluate the effects of different decellularization methods on the properties of genipin-cross-linked decellularized tracheal matrices in rabbits.
methodsThe tracheas of New Zealand rabbits were decellularized by the Triton-X 100-processed method (TPM) and the detergent-enzymatic method (DEM) and were then cross-linked with genipin. Mechanical tests, haematoxylin-eosin staining, Masson trichrome staining, Safranin O staining, DAPI staining, scanning electronic microscopy (SEM), and biocompatibility tests were used to evaluate the treatment. The bioengineered trachea and control trachea were then implanted into allogeneic rabbits for 30 days. The structural and functional analyses were performed after transplantation.
resultsThe biomechanical tests demonstrated that the biomechanical properties of the decellularized tracheas decreased and that genipin improved them (
conclusionsCompared with the trachea treated with the TPM, the rabbit trachea processed by the DEM had better biocompatibility and lower immunogenicity, and its structural and mechanical characteristics were effectively improved after the genipin treatment, which is suitable for engineering replacement tracheal tissue.
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