ArticleCellular & molecular biology letters2019
Reliable reference genes for expression analysis of proliferating and adipogenically differentiating human adipose stromal cells.
Article in Cellular & molecular biology letters, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- A comparative assessment of reference genes in mouse brown adipocyte differentiation and thermogenesis in vitro.Adipocyte · 2024Article
- Identification of the Reference Genes for Relative qRT-PCR Assay in Two Experimental Models of Rabbit and Horse Subcutaneous ASCs.International journal of molecular sciences · 2024Article
- Inflammation and Starvation Affect Housekeeping Gene Stability in Adipose Mesenchymal Stromal Cells.Current issues in molecular biology · 2024Article
- LRP10, PGK1 and RPLP0: Best Reference Genes in Periprostatic Adipose Tissue under Obesity and Prostate Cancer Conditions.International journal of molecular sciences · 2023Article
- Functional differentiation of the ovine preadipocytes -insights from gene expression profiling.Functional & integrative genomics · 2023Article
- The circadian transcription factor ARNTL2 is regulated by weight-loss interventions in human white adipose tissue and inhibits adipogenesis.Cell death discovery · 2022Article
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- Hypertrophy of Adipose Tissues in Quail Embryos byFrontiers in physiology · 2021Article
- Reporting Guidelines, Review of Methodological Standards, and Challenges Toward Harmonization in Bone Marrow Adiposity Research. Report of the Methodologies Working Group of the International Bone Marrow Adiposity Society.Frontiers in endocrinology · 2020Review
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Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe proliferation and adipogenic differentiation of adipose stromal cells (ASCs) are complex processes comprising major phenotypical alterations driven by up- and downregulation of hundreds of genes. Quantitative RT-PCR can be employed to measure relative changes in the expression of a gene of interest. This approach requires constitutively expressed reference genes for normalization to counteract inter-sample variations due to differences in RNA quality and quantity. Thus, a careful validation of quantitative RT-PCR reference genes is needed to accurately measure fluctuations in the expression of genes. Here, we evaluated candidate reference genes applicable for quantitative RT-PCR analysis of gene expression during proliferation and adipogenesis of human ASCs with the immunophenotype DLK1
methodsWe evaluated the applicability of 10 candidate reference genes (
resultsThe results indicate that
conclusionsApplying these findings for future gene expression analyses will help elucidate ASC biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.