ArticleFrontiers in neuroanatomy2019
iDISCO+ for the Study of Neuroimmune Architecture of the Rat Auditory Brainstem.
Article in Frontiers in neuroanatomy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Three-dimensional modeling of sensory nerve architecture and eosinophil and mast cell interactions in eosinophilic gastrointestinal disease.Journal of leukocyte biology · 2026Article
- Causal cross-trait mapping at single-cell resolution identifies shared immunogenetic drivers of migraine and Meniere's disease.The journal of headache and pain · 2026Article
- Reuniting and Endolymphatic Duct Macrophages: Localization and Possible Roles.Audiology research · 2025Article
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- ChFluids and barriers of the CNS · 2024Article
- Temporal bone marrow of the rat and its connections to the inner ear.Frontiers in neurology · 2024Article
- Multidimensional Imaging of Breast Cancer.Cold Spring Harbor perspectives in medicine · 2023Review
- Deconstructing the functional neuroanatomy of the choroid plexus: an ontogenetic perspective for studying neurodevelopmental and neuropsychiatric disorders.Molecular psychiatry · 2022Review
- HYBRiD: hydrogel-reinforced DISCO for clearing mammalian bodies.Nature methods · 2022Article
- Tissue clearing and 3D imaging in developmental biology.Development (Cambridge, England) · 2021Review
- Tutorial: practical considerations for tissue clearing and imaging.Nature protocols · 2021Review
- In Situ 3D-Imaging of the Inner Ear Synapses with a Cochlear Implant.Life (Basel, Switzerland) · 2021Article
- 3D Reconstruction of the Clarified Rat Hindbrain Choroid Plexus.Frontiers in cell and developmental biology · 2021Article
- Mast Cells in the Auditory Periphery of Rodents.Brain sciences · 2020Article
- Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease.iScience · 2020Review
- Cannabinoids, Inner Ear, Hearing, and Tinnitus: A Neuroimmunological Perspective.Frontiers in neurology · 2020Review
- Anti-inflammatory and Oto-Protective Effect of the Small Heat Shock Protein Alpha B-Crystallin (HspB5) in Experimental Pneumococcal Meningitis.Frontiers in neurology · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The lower stations of the auditory system display a complex anatomy. The inner ear labyrinth is composed of several interconnecting membranous structures encased in cavities of the temporal bone, and the cerebellopontine angle contains fragile structures such as meningeal folds, the choroid plexus (CP), and highly variable vascular formations. For this reason, most histological studies of the auditory system have either focused on the inner ear or the CNS by physically detaching the temporal bone from the brainstem. However, several studies of neuroimmune interactions have pinpointed the importance of structures such as meninges and CP; in the auditory system, an immune function has also been suggested for inner ear structures such as the endolymphatic duct (ED) and sac. All these structures are thin, fragile, and have complex 3D shapes. In order to study the immune cell populations located on these structures and their relevance to the inner ear and auditory brainstem in health and disease, we obtained a clarified-decalcified preparation of the rat hindbrain still attached to the intact temporal bone. This preparation may be immunolabeled using a clearing protocol (based on iDISCO+) to show location and functional state of immune cells. The observed macrophage distribution suggests the presence of CP-mediated communication pathways between the inner ear and the cochlear nuclei.
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