ArticleTurkish journal of biology = Turk biyoloji dergisi2018
Ultrasound-enhanced gene delivery to alfalfa cells by hPAMAM dendrimer nanoparticles.
Article in Turkish journal of biology = Turk biyoloji dergisi, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- The role of nanoparticles in transforming plant genetic engineering: advancements, challenges and future prospects.Functional & integrative genomics · 2025Review
- Transport of Nanoparticles into Plants and Their Detection Methods.Nanomaterials (Basel, Switzerland) · 2024Review
- Perspectives on new opportunities for nano-enabled strategies for gene delivery to plants using nanoporous materials.Planta · 2021Review
- Application of naturally occurring mechanical forces inPlant signaling & behavior · 2021Review
- Nanoscale Drug Delivery Systems: From Medicine to Agriculture.Frontiers in bioengineering and biotechnology · 2020Review
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cationic polyamidoamine (PAMAM) dendrimers are highly branched nanoparticles with unique molecular properties, which make them promising nanocarriers for gene delivery into cells. This research evaluated the ability of hyperbranched PAMAM (hPAMAM)-G2 with a diethylenetriamine core to interact with DNA, its protection from ultrasonic damage, and delivery to alfalfa cells. Additionally, the effects of ultrasound on the efficacy of hPAMAM-G2 for the delivery and expression of the gus A gene in the alfalfa cells were investigated. The electrophoresis retardation of plasmid DNA occurred at an N/P ratio (where N is the number of hPAMAM nitrogen atoms and P is the number of DNA phosphorus atoms) of 3 and above, and hPAMAM-G2 dendrimers completely immobilized the DNA at an N/P ratio of 4. The analysis of the DNA dissociated from the dendriplexes revealed a partial protection of the DNA from ultrasound damage at N/P ratios lower than 2, and with increasing N/P ratios, the DNA was better protected. Sonication of the alfalfa cells in the presence of ssDNA-FITC-hPAMAM increased the ssDNA delivery efficiency to 36%, which was significantly higher than that of ssDNA-FITC-hPAMAM without sonication. Additionally, the efficiency of transfection and the expression of the gus A gene were dependent on the N/P ratio and the highest efficiency (1.4%) was achieved at an N/P ratio of 10. The combination of 120 s of ultrasound and hPAMAM-DNA increased the gusA gene transfection and expression to 3.86%.
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