ArticleScientific reports2019
Insulin secretory granules labelled with phogrin-fluorescent proteins show alterations in size, mobility and responsiveness to glucose stimulation in living β-cells.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 31 citations in OpenAlex.
- Secretory stimuli distinctly regulate insulin secretory granule maturation through structural remodeling.Structure (London, England : 1993) · 2025Article
- A Comprehensive Workflow for Imaging Live Insulin Secretion Events and Granules in Intact Islets.bioRxiv : the preprint server for biology · 2025Article
- Measuring pH in insulin secretory granules by phasor-based fluorescence lifetime imaging of a genetically encoded sensor.Communications biology · 2025Article
- Decoding Insulin Secretory Granule Maturation Using Genetically Encoded pH Sensors.ACS sensors · 2024Article
- Unveiling nanoscale optical signatures of cytokine-induced β-cell dysfunction.Scientific reports · 2023Article
- Molecular Research on Genes Involved in Metabolic Diseases.Biomedicines · 2023Article
- Single-molecule analysis of intracellular insulin granule behavior and its application to analyzing cytoskeletal dependence and pathophysiological implications.Frontiers in physiology · 2023Article
- Single-cell imaging of α and β cell metabolic response to glucose in living human Langerhans islets.Communications biology · 2022Article
- Measuring Molecular Diffusion in Dynamic Subcellular Nanostructures by Fast Raster Image Correlation Spectroscopy and 3D Orbital Tracking.International journal of molecular sciences · 2022Article
- The changing view of insulin granule mobility: From conveyor belt to signaling hub.Frontiers in endocrinology · 2022Review
- Sweet Taste Preference: Relationships with Other Tastes, Liking for Sugary Foods and Exploratory Genome-Wide Association Analysis in Subjects with Metabolic Syndrome.Biomedicines · 2021Article
- β-Cell Pathophysiology: A Review of Advanced Optical Microscopy Applications.International journal of molecular sciences · 2021Review
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- Article
- Isolation and Proteomics of the Insulin Secretory Granule.Metabolites · 2021Review
- Conventional and Unconventional Mechanisms by which Exocytosis Proteins Oversee β-cell Function and Protection.International journal of molecular sciences · 2021Review
- Determination of secretory granule maturation times in pancreatic islet β-cells by serial block-face electron microscopy.Journal of structural biology · 2020Article
- Article
- ZIGIR, a Granule-Specific ZnCell reports · 2020Article
- Metabolic response of Insulinoma 1E cells to glucose stimulation studied by fluorescence lifetime imaging.FASEB bioAdvances · 2020Article
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Authors and funding
10 authors at 6 institutions in 2 countries.
Funding
Abstract
The intracellular life of insulin secretory granules (ISGs) from biogenesis to secretion depends on their structural (e.g. size) and dynamic (e.g. diffusivity, mode of motion) properties. Thus, it would be useful to have rapid and robust measurements of such parameters in living β-cells. To provide such measurements, we have developed a fast spatiotemporal fluctuation spectroscopy. We calculate an imaging-derived Mean Squared Displacement (iMSD), which simultaneously provides the size, average diffusivity, and anomalous coefficient of ISGs, without the need to extract individual trajectories. Clustering of structural and dynamic quantities in a multidimensional parametric space defines the ISGs' properties for different conditions. First, we create a reference using INS-1E cells expressing proinsulin fused to a fluorescent protein (FP) under basal culture conditions and validate our analysis by testing well-established stimuli, such as glucose intake, cytoskeleton disruption, or cholesterol overload. After, we investigate the effect of FP-tagged ISG protein markers on the structural and dynamic properties of the granule. While iMSD analysis produces similar results for most of the lumenal markers, the transmembrane marker phogrin-FP shows a clearly altered result. Phogrin overexpression induces a substantial granule enlargement and higher mobility, together with a partial de-polymerization of the actin cytoskeleton, and reduced cell responsiveness to glucose stimulation. Our data suggest a more careful interpretation of many previous ISG-based reports in living β-cells. The presented data pave the way to high-throughput cell-based screening of ISG structure and dynamics under various physiological and pathological conditions.
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