Evidence map›Paper›PMID 30811771›Full record

ArticleStem cells (Dayton, Ohio)2019

Highly Purified Human Extracellular Vesicles Produced by Stem Cells Alleviate Aging Cellular Phenotypes of Senescent Human Cells.

Senquan Liu, Vasiliki Mahairaki, Hao Bai, Zheng Ding, Jiaxin Li, Kenneth W Witwer, Linzhao Cheng

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells (Dayton, Ohio), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
105citing papers in PubMed, 1 pooled it
7.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

105 citing papers in PubMed, 1 synthesis or guideline pooled it, 163 citations in OpenAlex.

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45 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Senquan LiuDepartment of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Vasiliki MahairakiInstitute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Hao BaiDepartment of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Zheng DingDepartment of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Jiaxin LiInstitute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Kenneth W WitwerDepartment of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Linzhao ChengDepartment of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.ORCID 0000-0001-7052-0296
Johns Hopkins University · US

Funding

Discovering novel players in mechanisms of extracellular vesicle release, cargo loading, and early pathogenesis of late-onset Alzheimer's DiseaseR56AG057430 · NIA · JOHNS HOPKINS UNIVERSITY · PI CHENG, LINZHAO, MACHAIRAKI, VASILIKI · 2017 to 2017
$796k
NIA NIH HHS R56 AG057430NIH HHS R56 AG-057430
6 · The paper itself

Abstract

Extracellular vesicles (EVs), including exosomes and microvesicles, mediate intercellular communications and exert various biological activities via delivering unique cargos of functional molecules such as RNAs and proteins to recipient cells. Previous studies showed that EVs produced and secreted by human mesenchymal stem cells (MSCs) can substitute intact MSCs for tissue repair and regeneration. In this study, we examined properties and functions of EVs from human induced pluripotent stem cells (iPSCs) that can be cultured infinitely under a chemically defined medium free of any exogenous EVs. We collected and purified EVs secreted by human iPSCs and MSCs. Purified EVs produced by both stem cell types have similar sizes (∼150 nm in diameter), but human iPSCs produced 16-fold more EVs than MSCs. When highly purified iPSC-EVs were applied in culture to senescent MSCs that have elevated reactive oxygen species (ROS), human iPSC-EVs reduced cellular ROS levels and alleviated aging phenotypes of senescent MSCs. Our discovery reveals that EVs from human stem cells can alleviate cellular aging in culture, at least in part by delivering intracellular peroxiredoxin antioxidant enzymes. Stem Cells 2019;37:779-790.

Indexed as

AntioxidantsBiological TransportCell CommunicationCellular SenescenceExtracellular VesiclesGene ExpressionGenetic VectorsHumansInduced Pluripotent Stem CellsLamin Type ALentivirusMesenchymal Stem CellsPeroxiredoxinsPhenotypePrimary Cell CultureReactive Oxygen SpeciesAntioxidantsLamin Type APeroxiredoxinsprelamin AReactive Oxygen SpeciesAgingExtracellular vesiclesHuman induced pluripotent stem cellsMesenchymal stem cellsPeroxiredoxinSenescence

Identifiers

PMID30811771
PMCPMC6767364
OpenAlexW2917107183

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.