ArticleAngewandte Chemie (International ed. in English)2019
Lipid Heterogeneity between Astrocytes and Neurons Revealed by Single-Cell MALDI-MS Combined with Immunocytochemical Classification.
Article in Angewandte Chemie (International ed. in English), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
79 citing papers in PubMed, 119 citations in OpenAlex.
- Protocol for reconstructing 2D and 3D peptide atlases of the Ciona cerebral ganglion at single-cell resolution.STAR protocols · 2026Article
- Non-decalcified human teeth sample preparation method for MALDI mass spectrometry imaging.Analytica chimica acta · 2026Article
- Progress of metabolomics-centric multi-omics research in medicine.iMetaOmics · 2026Review
- Spatial Detection of Microsphere Polystyrene Plastics and Molecular Remodeling within a Full-Body Mouse with MALDI Trapped Ion Mobility Mass Spectrometry Imaging.Angewandte Chemie (International ed. in English) · 2026Article
- Roles of FXR in Lipid Biochemistry within Hepatocellular Carcinoma Using MALDI MSI.Journal of the American Society for Mass Spectrometry · 2026Article
- New Analytical Technologies to Resolve, Interpret, and Understand Lipid Complexity.Annual review of analytical chemistry (Palo Alto, Calif.) · 2026Review
- Proteoform profiling of endogenous single cells from rat hippocampus at scale.Nature biotechnology · 2026Article
- Deep-coverage single-cell metabolomics enabled by ion mobility-resolved mass cytometry.Nature methods · 2026Article
- Characterization of the Lipidome of Neurons in Mouse Brain Nuclei Using Imaging Mass Spectrometry.Analytical chemistry · 2026Article
- Multimodal mass spectrometry imaging for plaque- and region-specific neurolipidomics in Alzheimer's disease mouse models.Nature communications · 2025Article
- Multi-modal single-cell platform for nanoparticle-enhanced time-series metabolic profiles of CD8Journal of nanobiotechnology · 2025Article
- Two- and three-dimensional neuropeptidomic landscape in the central nervous system of an invertebrate chordate,iScience · 2025Article
- High-Throughput Fluorescence-Guided Sequential Single-Cell MALDI-ICC Mass Spectrometry.Analytical chemistry · 2025Article
- Structure Elucidation for MALDI Mass Spectrometry Imaging Using Infrared Ion Spectroscopy.Analytical chemistry · 2025Article
- Spatial Multiomics Toward Understanding Neurological Systems.Journal of mass spectrometry : JMS · 2025Review
- Fluorescence-Guided Matrix-assisted Laser Desorption/Ionization with Laser-Induced Postionization Mass Spectrometry of Individual Rat Neural Cells.Journal of visualized experiments : JoVE · 2025Article
- Recent Developments in Single-Cell Metabolomics by Mass Spectrometry─A Perspective.Journal of proteome research · 2025Review
- Resolving multi-image spatial lipidomic responses to inhaled toxicants by machine learning.Nature communications · 2025Article
- Single-cell lipidomics: protocol development for reliable cellular profiling using capillary sampling.The Analyst · 2025Article
- Review
19 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Transcriptomics characterizes cells based on their potential molecular repertoire whereas direct mass spectrometry (MS) provides information on the actual compounds present within cells. Single-cell matrix-assisted laser desorption/ionization (MALDI) MS can measure the chemical contents of individual cells but spectra are difficult to correlate to conventional cell types, limiting the metabolic information obtained. We present a protocol that combines MALDI-MS with immunocytochemistry to assay over a thousand individual rat brain cells. The approach entwines the wealth of knowledge obtained by immunocytochemical profiling with mass spectral information on the predominant lipids within each cell. While many lipid species showed a high degree of similarity between neurons and astrocytes, seventeen significantly differed between the two cell types, including four phosphatidylethanolamines elevated in astrocytes and nine phosphatidylcholines in neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.