ArticlePloS one2019
Modulation of microbial communities and mucosal gene expression in chicken intestines after galactooligosaccharides delivery In Ovo.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 85 citations in OpenAlex.
- Low-pass whole-genome sequencing reveals genomic diversity and ecotype-specific adaptation in indigenous Tigrayan chickens.Scientific reports · 2026Article
- Effect of using mannan-oligosaccharides and β-glucans as feed additives on growth, biochemical parameters, and immunity of Salmonella enterica serovar Enteritidis-challenged turkey.Veterinary research communications · 2026Article
- Transcriptional Cytokine Responses Linked to Pathological Outcomes in Broiler Chickens Fed Residual Doses of Antimicrobials.International journal of molecular sciences · 2026Article
- The effect of in ovo stimulation with oligosaccharides on oxidative stress markers, enzymatic activity, and mucin concentration in the intestinal mucosa of broiler chickens.BMC veterinary research · 2026Article
- Maternal curcumin supplementation alleviates intestinal inflammation ofAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Article
- Synbiotics: An Emerging Frontier in Infectious Diseases Control.Probiotics and antimicrobial proteins · 2026Review
- Alginate oligosaccharides derived from tropical brown seaweeds as sustainable alternatives to antibiotic growth promoters in poultry nutrition: Functional mechanisms and production perspectives.Veterinary world · 2026Review
- Modulatory effects of in ovo delivery of galactooligosaccharide and Lactiplantibacillus plantarum on antioxidant capacity, gene expression, and selected plasma metabolite parameters of broiler chickens.Journal of applied genetics · 2025Article
- Effects of Lactiplantibacillus plantarum and Galactooligosaccharide Administered In Ovo on Hatchability, Chick Quality, Performance, Caecal Histomorphology and Meat Quality Traits of Broiler Chickens.Journal of animal physiology and animal nutrition · 2025Article
- Modulation of gene expression in immune-related organs by in ovo stimulation with probiotics and prophybiotics in broiler chickens.Journal of applied genetics · 2025Article
- Inter- and Transgenerational Effects of In Ovo Stimulation with Bioactive Compounds on Cecal Tonsils and Cecal Mucosa Transcriptomes in a Chicken Model.International journal of molecular sciences · 2025Article
- Epigenetic changes in pyloric caeca of Atlantic salmon fed diets containing increasing levels of lipids and choline.Epigenetics · 2024Article
- Article
- Inclusion of slowly digestible starch source is a promising strategy than reducing starch to protein ratio in low protein broiler diets.Poultry science · 2024Article
- Genomic analysis of Nigerian indigenous chickens reveals their genetic diversity and adaptation to heat-stress.Scientific reports · 2024Article
- Expression of IL-1β in transgenicFrontiers in immunology · 2024Article
- State of the art in research on the gut-liver and gut-brain axis in poultry.Journal of animal science and biotechnology · 2023Review
- MicroRNA expression in immune tissues of adult chickens after embryo stimulation with bioactive substances.Scientific reports · 2023Article
- Physiological effects ofFrontiers in veterinary science · 2023Review
- Maternal stevioside supplementation improves intestinal immune function of chicken offspring potentially via modulating gut microbiota and down-regulating the promoter methylation level of suppressor of cytokine signaling 1 (Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intestinal mucosa is the interface between the microbial content of the gut and the host's milieu. The goal of this study was to modulate chicken intestinal microflora by in ovo stimulation with galactooligosaccharides (GOS) prebiotic and to demonstrate the molecular responses of the host. The animal trial was performed on meat-type chickens (Ross 308). GOS was delivered by in ovo injection performed into the air cell on day 12 of egg incubation. Analysis of microbial communities and mucosal gene expression was performed at slaughter (day 42 post-hatching). Chyme (for DNA isolation) and intestinal mucosa (for RNA isolation) from four distinct intestinal segments (duodenum, jejunum, ileum, and caecum) was sampled. The relative abundance of Bifidobacterium spp. and Lactobacillus spp. in DNA isolated from chyme samples was determined using qPCR. On the host side, the mRNA expression of 13 genes grouped into two panels was analysed with RT-qPCR. Panel (1) included genes related to intestinal innate immune responses (IL-1β, IL-10 and IL-12p40, AvBD1 and CATHL2). Panel (2) contained genes involved in intestinal barrier function (MUC6, CLDN1 and TJAP1) and nutrients sensing (FFAR2 and FFAR4, GLUT1, GLUT2 and GLUT5). GOS increased the relative abundance of Bifidobacterium in caecum (from 1.3% to 3.9%). Distinct effects of GOS on gene expression were manifested in jejunum and caecum. Cytokine genes (IL-1β, IL-10 and IL-12p40) were up-regulated in the jejunum and caecum of the GOS-treated group. Host defence peptides (AvBD1 and CATHL2) were up-regulated in the caecum of the GOS-treated group. Free fatty acid receptors (FFAR2 and FFAR4) were up-regulated in all three compartments of the intestine (except the duodenum). Glucose transporters were down-regulated in duodenum (GLUT2 and GLUT5) but up-regulated in the hindgut (GLUT1 and GLUT2). In conclusion, GOS delivered in ovo had a bifidogenic effect in adult chickens. It also modulated gene expression related to intestinal immune responses, gut barrier function, and nutrient sensing.
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