ArticlePloS one2019
Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Which Factors Affect the Occurrence of Off-Target Effects Caused by the Use of CRISPR/Cas: A Systematic Review in Plants.Frontiers in plant science · 2020Pooled it
- Medicinal-plant immunity and specialized metabolism in plant-pathogen interactions: mechanisms and applications.Planta · 2026Review
- dSaCas9 enables enhanced transcriptional activation in Nicotiana benthamiana compared to its dSpCas9 ortholog.BMC plant biology · 2026Article
- A long journey towards genome editing technologies in plants: a technical and critical review of genome editing technologies.Frontiers in genome editing · 2025Review
- How to use CRISPR/Cas9 in plants: from target site selection to DNA repair.Journal of experimental botany · 2024Review
- Comparison of Cas12a and Cas9-mediated mutagenesis in tomato cells.Scientific reports · 2024Article
- CRISPR/Cas9 as a Mutagenic Factor.International journal of molecular sciences · 2024Review
- An extensible vector toolkit and parts library for advanced engineering of plant genomes.The plant genome · 2023Article
- DNA rehybridization drives product release from Cas9 ribonucleoprotein to enable multiple-turnover cleavage.Nucleic acids research · 2023Article
- High-throughput sgRNA testing reveals rules for Cas9 specificity and DNA repair in tomato cells.Frontiers in genome editing · 2023Article
- A User's Guide to Golden Gate Cloning Methods and Standards.ACS synthetic biology · 2022Review
- CRISPR/Cas9 and Nanotechnology Pertinence in Agricultural Crop Refinement.Frontiers in plant science · 2022Review
- Cas9-Mediated Targeted Mutagenesis in Plants.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Molecular and Computational Strategies to Increase the Efficiency of CRISPR-Based Techniques.Frontiers in plant science · 2022Review
- A CRISPR way for accelerating cereal crop improvement: Progress and challenges.Frontiers in genetics · 2022Review
- Novel CRISPR/Cas applications in plants: from prime editing to chromosome engineering.Transgenic research · 2021Review
- Efficient gene targeting in Nicotiana tabacum using CRISPR/SaCas9 and temperature tolerant LbCas12a.Plant biotechnology journal · 2021Article
- Rational design of minimal synthetic promoters for plants.Nucleic acids research · 2020Article
- Review
- Quantification of the affinities of CRISPR-Cas9 nucleases for cognate protospacer adjacent motif (PAM) sequences.The Journal of biological chemistry · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Molecular tools adapted from bacterial CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats) systems for adaptive immunity have become widely used for plant genome engineering, both to investigate gene functions and to engineer desirable traits. A number of different Cas (CRISPR-associated) nucleases are now used but, as most studies performed to date have engineered different targets using a variety of plant species and molecular tools, it has been difficult to draw conclusions about the comparative performance of different nucleases. Due to the time and effort required to regenerate engineered plants, efficiency is critical. In addition, there have been several reports of mutations at sequences with less than perfect identity to the target. While in some plant species it is possible to remove these so-called 'off-targets' by backcrossing to a parental line, the specificity of genome engineering tools is important when targeting specific members of closely-related gene families, especially when recent paralogues are co-located in the genome and unlikely to segregate. Specificity is also important for species that take years to reach sexual maturity or that are clonally propagated. Here, we directly compare the efficiency and specificity of Cas nucleases from different bacterial species together with engineered variants of Cas9. We find that the nucleotide content of the target correlates with efficiency and that Cas9 from Staphylococcus aureus (SaCas9) is comparatively most efficient at inducing mutations. We also demonstrate that 'high-fidelity' variants of Cas9 can reduce off-target mutations in plants. We present these molecular tools as standardised DNA parts to facilitate their re-use.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.