ArticlePLoS biology2019
A determining factor for insect feeding preference in the silkworm, Bombyx mori.
Article in PLoS biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed, 144 citations in OpenAlex.
- The lepidopteran analyst: how caterpillars, moths and butterflies encode taste identity and valence.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2026Review
- CRISPR-Mediated Silkworm: The Oncoming Agricultural Revolutions and a Rising Model Organism.Genes · 2026Review
- Convergence of alimentary air inflation and adult non-feeding in insects, and possible adaptive functions.PloS one · 2026Article
- Geographic and seasonal variation of culturable bacteria associated with the diseased silkworm (Current research in microbial sciences · 2026Article
- Article
- Starvation During the Larval Stage Driving Population Decline in the Butterfly SpecialistInsects · 2025Article
- Gustatory receptors interact with the serotonin pathway to regulate silkmoth stereotyped courtship behavior.BMC biology · 2025Article
- Identification and Expression Profiles of Chemosensory Genes in the Antennal Transcriptome ofInsects · 2025Article
- Article
- Population Dynamics ofInsects · 2025Article
- Molecular evolution of dietary shifts in ladybird beetles (Coleoptera: Coccinellidae): from fungivory to carnivory and herbivory.BMC biology · 2025Article
- Harnessing Insect Chemosensory and Mechanosensory Receptors Involved in Feeding for Precision Pest Management.Life (Basel, Switzerland) · 2025Review
- Genetic Diversity and Differentiation of Silkworm (Insects · 2024Article
- Larval Feeding Habits of Five Firefly Species Across Aquatic, Semi-Aquatic, and Terrestrial Lineages.Insects · 2024Article
- Molecular Functions and Physiological Roles of Gustatory Receptors of the SilkwormInternational journal of molecular sciences · 2024Review
- Gustatory receptor 11 is involved in detecting the oviposition water of Asian tiger mosquito, Aedes albopictus.Parasites & vectors · 2024Article
- Population genomics of Agrotis segetum provide insights into the local adaptive evolution of agricultural pests.BMC biology · 2024Article
- Effects of Habitual Dietary Change on the Gut Microbiota and Health of Silkworms.International journal of molecular sciences · 2024Article
- Analysis of Genes Associated with Feeding Preference and Detoxification in Various Developmental Stages ofInsects · 2024Article
- Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control inInsects · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
Feeding preference is critical for insect adaptation and survival. However, little is known regarding the determination of insect feeding preference, and the genetic basis is poorly understood. As a model lepidopteran insect with economic importance, the domesticated silkworm, Bombyx mori, is a well-known monophagous insect that predominantly feeds on fresh mulberry leaves. This species-specific feeding preference provides an excellent model for investigation of host-plant selection of insects, although the molecular mechanism underlying this phenomenon remains unknown. Here, we describe the gene GR66, which encodes a putative bitter gustatory receptor (GR) that is responsible for the mulberry-specific feeding preference of B. mori. With the aid of a transposon-based, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) system, the GR66 locus was genetically mutated, and homozygous mutant silkworm strains with truncated gustatory receptor 66 (GR66) proteins were established. GR66 mutant larvae acquired new feeding activity, exhibiting the ability to feed on a number of plant species in addition to mulberry leaves, including fresh fruits and grain seeds that are not normally consumed by wild-type (WT) silkworms. Furthermore, a feeding choice assay revealed that the mutant larvae lost their specificity for mulberry. Overall, our findings provide the first genetic and phenotypic evidences that a single bitter GR is a major factor affecting the insect feeding preference.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.