Evidence map›Paper›PMID 30810117›Full record

ArticleMedical science monitor : international medical journal of experimental and clinical research2019

Long Chain Non-Coding RNA (lncRNA) HOTAIR Knockdown Increases miR-454-3p to Suppress Gastric Cancer Growth by Targeting STAT3/Cyclin D1.

Datong Jiang, He Li, Heping Xiang, Ming Gao, Chunlin Yin, Haiping Wang, Yuansong Sun, Maoming Xiong

RetractedOpen access · hybridAbstract readRetracted Publication
PubMed Full text at PMC Publisher
In one paragraph

Article in Medical science monitor : international medical journal of experimental and clinical research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 25 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed, 1 pooled it
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.

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  5. Retracted: Long Chain Non-Coding RNA (lncRNA) HOTAIR Knockdown Increases miR-454-3p to Suppress Gastric Cancer Growth by Targeting STAT3/Cyclin D1.Medical science monitor : international medical journal of experimental and clinical research · 2023
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Datong JiangDepartment of Emergency Surgery, Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China (mainland).
He LiDepartment of Emergency Surgery, Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China (mainland).
Heping XiangDepartment of Emergency Surgery, Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China (mainland).
Ming GaoDepartment of Emergency Surgery, Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China (mainland).
Chunlin YinDepartment of Emergency Surgery, Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China (mainland).
Haiping WangDepartment of Emergency Surgery, Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China (mainland).
Yuansong SunDepartment of Emergency Surgery, Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China (mainland).
Maoming XiongDepartment of Gastroenterological Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China (mainland).
Second Hospital of Anhui Medical University · CNAnhui Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND Gastric cancer is a common gastrointestinal tumor. The incidence and mortality of gastric cancer are very high. Therefore, it is important to study targeted drugs. Recent studies found long chain non-coding RNA (lncRNAs) and microRNAs (miRNAs) were abnormal in gastric cancer. MATERIAL AND METHODS We collected adjacent normal and cancer tissues of gastric cancer patients and measured HOTAIR, miR-454-3p, STAT3, and Cyclin D1 expression and analyzed the correlation with clinical status. We also measured AGS and SGC7901 cells proliferation rate of different groups by MTT assay, and we evaluated AGS and SGC7901 cell apoptosis and cell cycle by flow cytometry. In addition, we assessed the relative proteins expressions by WB assay. Finally, we explored the correlation between miR-454-3p and STAT3 by use of double luciferase reporter. RESULTS lncRNA HOTAIR was negatively correlated with miR-454-3p expression in gastric cancer tissues. lncRNA HOTAIR knockdown suppressed AGS and SGC7901, which are gastric cancer cell lines that promote cell proliferation by increasing cell apoptosis and keeping the cell cycle in G1 phase. In further mechanism research, we found that the STAT3 and Cyclin D1 proteins expressions were suppressed by lncRNA HOTAIR down-regulation in AGS and SGC7901 cells. CONCLUSIONS Our results suggest that lncRNA HOTAIR knockdown stimulates miR-454-3p expression to inhibit gastric cancer growth by depressing STAT3/Cyclin D1 activity.

Indexed as

ApoptosisCell Line, TumorCell MovementCell ProliferationCyclin D1Gene Knockdown TechniquesGenes, bcl-1HumansMicroRNAsRNA, Long NoncodingSTAT3 Transcription FactorStomach NeoplasmsCCND1 protein, humanCyclin D1HOTAIR long untranslated RNA, humanMicroRNAsMIRN454 microRNA, humanRNA, Long NoncodingSTAT3 protein, humanSTAT3 Transcription Factor

Identifiers

PMID30810117
PMCPMC6402277
OpenAlexW2916678593

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.