ArticleThe Plant journal : for cell and molecular biology2019
MyROOT: a method and software for the semiautomatic measurement of primary root length in Arabidopsis seedlings.
Article in The Plant journal : for cell and molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 40 citations in OpenAlex.
- Evaluation of soybean sprouting growth vigor based on ZnONPs.Frontiers in plant science · 2026Article
- Automatic root measurement: a lightweight method for measuring pea root length.Plant methods · 2025Article
- LenRuler: a rice-centric method for automated radicle length measurement with multicrop validation.Plant phenomics (Washington, D.C.) · 2025Article
- Improved Transformer for Time Series Senescence Root Recognition.Plant phenomics (Washington, D.C.) · 2024Article
- Root Walker: an automated pipeline for large scale quantification of early root growth responses at high spatial and temporal resolution.The Plant journal : for cell and molecular biology · 2024Article
- Iterative image segmentation of plant roots for high-throughput phenotyping.Scientific reports · 2022Article
- Recent advances in methods forPeerJ · 2022Review
- The intrinsic chaperone network of Arabidopsis stem cells confers protection against proteotoxic stress.Aging cell · 2021Article
- Precise transcriptional control of cellular quiescence by BRAVO/WOX5 complex in Arabidopsis roots.Molecular systems biology · 2021Article
- Dissecting the Root Phenotypic and Genotypic Variability of the Iowa Mung Bean Diversity Panel.Frontiers in plant science · 2021Article
- HyLength: a semi-automated digital image analysis tool for measuring the length of roots and fungal hyphae of dense mycelia.Mycorrhiza · 2020Article
- The platformPlant methods · 2020Article
- High-ThroughputFrontiers in plant science · 2020Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Root analysis is essential for both academic and agricultural research. Despite the great advances in root phenotyping and imaging, calculating root length is still performed manually and involves considerable amounts of labor and time. To overcome these limitations, we developed MyROOT, a software for the semiautomatic quantification of root growth of seedlings growing directly on agar plates. Our method automatically determines the scale from the image of the plate, and subsequently measures the root length of the individual plants. To this aim, MyROOT combines a bottom-up root tracking approach with a hypocotyl detection algorithm. At the same time as providing accurate root measurements, MyROOT also significantly minimizes the user intervention required during the process. Using Arabidopsis, we tested MyROOT with seedlings from different growth stages and experimental conditions. When comparing the data obtained from this software with that of manual root measurements, we found a high correlation between both methods (R
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