ArticlemBio2019
Large Circular Plasmids from Groundwater Plasmidomes Span Multiple Incompatibility Groups and Are Enriched in Multimetal Resistance Genes.
Article in mBio, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 56 citations in OpenAlex.
- Only intercalating ions cause the temperature sensitivity in acridine orange-stained artificial nine-plasmid system-2.Scientific reports · 2026Article
- Origin of replication discovery for environmentally isolatediScience · 2026Article
- Megaplasmids associate withbioRxiv : the preprint server for biology · 2025Article
- Unveiling plasmid diversity and functionality in pristine groundwater.Environmental microbiome · 2025Article
- The influence of depth on the global deep-sea plasmidome.Scientific reports · 2025Article
- Genetic connections and antimicrobial resistance in dogs and ownersFrontiers in veterinary science · 2025Article
- Article
- Unravelling the mechanisms of antibiotic and heavy metal resistance co-selection in environmental bacteria.FEMS microbiology reviews · 2024Review
- PlasmidHunter: accurate and fast prediction of plasmid sequences using gene content profile and machine learning.Briefings in bioinformatics · 2024Article
- Article
- Mixed waste contamination selects for a mobile genetic element population enriched in multiple heavy metal resistance genes.ISME communications · 2024Article
- Characterization of the Environmental Plasmidome of the Red Sea.Microbiology spectrum · 2023Article
- A mathematician's guide to plasmids: an introduction to plasmid biology for modellers.Microbiology (Reading, England) · 2023Review
- Efficient Suppression of Natural Plasmid-Borne Gene Expression in Carbapenem-Resistant Klebsiella pneumoniae Using a Compact CRISPR Interference System.Antimicrobial agents and chemotherapy · 2022Article
- Delivering the Promise of Gene Therapy with Nanomedicines in Treating Central Nervous System Diseases.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Inter-species geographic signatures for tracing horizontal gene transfer and long-term persistence of carbapenem resistance.Genome medicine · 2022Article
- Genotype to ecotype in niche environments: adaptation of Arthrobacter to carbon availability and environmental conditions.ISME communications · 2022Article
- Assessment of the plasmidome of an extremophilic microbial community from the Diamante Lake, Argentina.Scientific reports · 2021Article
- A Peek into the Plasmidome of Global Sewage.mSystems · 2021Article
- Overview of bioinformatic methods for analysis of antibiotic resistome from genome and metagenome data.Journal of microbiology (Seoul, Korea) · 2021Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Naturally occurring plasmids constitute a major category of mobile genetic elements responsible for harboring and transferring genes important in survival and fitness. A targeted evaluation of plasmidomes can reveal unique adaptations required by microbial communities. We developed a model system to optimize plasmid DNA isolation procedures targeted to groundwater samples which are typically characterized by low cell density (and likely variations in the plasmid size and copy numbers). The optimized method resulted in successful identification of several hundred circular plasmids, including some large plasmids (11 plasmids more than 50 kb in size, with the largest being 1.7 Mb in size). Several interesting observations were made from the analysis of plasmid DNA isolated in this study. The plasmid pool (plasmidome) was more conserved than the corresponding microbiome distribution (16S rRNA based). The circular plasmids were diverse as represented by the presence of seven plasmid incompatibility groups. The genes carried on these groundwater plasmids were highly enriched in metal resistance. Results from this study confirmed that traits such as metal, antibiotic, and phage resistance along with toxin-antitoxin systems are encoded on abundant circular plasmids, all of which could confer novel and advantageous traits to their hosts. This study confirms the ecological role of the plasmidome in maintaining the latent capacity of a microbiome, enabling rapid adaptation to environmental stresses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.