ArticleLife science alliance2019
Main constraints for RNAi induced by expressed long dsRNA in mouse cells.
Article in Life science alliance, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Small RNA pathways in mammalian oocytes.FEBS open bio · 2026Review
- Characterization of RNA interference in the cnidarian Nematostella vectensis reveals partial target silencing but lack of small RNA amplification.PLoS biology · 2026Article
- Response to "Antiviral RNA silencing in mammals: the importance of selecting the appropriate experimental model".Nucleic acids research · 2025Article
- Enhanced RNAi does not provide efficient innate antiviral immunity in mice.Nucleic acids research · 2025Article
- Functional canonical RNAi in mice expressing a truncated Dicer isoform and long dsRNA.EMBO reports · 2024Article
- AGO2 silences mobile transposons in the nucleus of quiescent cells.Nature structural & molecular biology · 2023Article
- Structural and functional basis of mammalian microRNA biogenesis by Dicer.Molecular cell · 2022Article
- STUB1 regulates antiviral RNAi through inducing ubiquitination and degradation of Dicer and AGO2 in mammals.Virologica Sinica · 2022Article
- Physiologically relevant miRNAs in mammalian oocytes are rare and highly abundant.EMBO reports · 2022Article
- Article
- The most abundant maternal lncRNA Sirena1 acts post-transcriptionally and impacts mitochondrial distribution.Nucleic acids research · 2020Article
- Feeding exogenous dsRNA interferes with endogenous sRNA accumulation in Paramecium.DNA research : an international journal for rapid publication of reports on genes and genomes · 2020Article
- Key Mechanistic Principles and Considerations Concerning RNA Interference.Frontiers in plant science · 2020Review
- Restricted and non-essential redundancy of RNAi and piRNA pathways in mouse oocytes.PLoS genetics · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNAi is the sequence-specific mRNA degradation guided by siRNAs produced from long dsRNA by RNase Dicer. Proteins executing RNAi are present in mammalian cells but rather sustain the microRNA pathway. Aiming for a systematic analysis of mammalian RNAi, we report here that the main bottleneck for RNAi efficiency is the production of functional siRNAs, which integrates Dicer activity, dsRNA structure, and siRNA targeting efficiency. Unexpectedly, increased expression of Dicer cofactors TARBP2 or PACT reduces RNAi but not microRNA function. Elimination of protein kinase R, a key dsRNA sensor in the interferon response, had minimal positive effects on RNAi activity in fibroblasts. Without high Dicer activity, RNAi can still occur when the initial Dicer cleavage of the substrate yields an efficient siRNA. Efficient mammalian RNAi may use substrates with some features of microRNA precursors, merging both pathways even more than previously suggested. Although optimized endogenous Dicer substrates mimicking miRNA features could evolve for endogenous regulations, the same principles would make antiviral RNAi inefficient as viruses would adapt to avoid efficacy.
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