Evidence map›Paper›PMID 30808331›Full record

ArticleBMC musculoskeletal disorders2019

Investigating the effect of proteoglycan 4 on hyaluronan solution properties using confocal fluorescence recovery after photobleaching.

Adam K Bloom, Michael L Samsom, Suresh C Regmi, Bridgett L Steele, Tannin A Schmidt

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC musculoskeletal disorders, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 11 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 2 countries.

Adam K BloomBiomedical Engineering Graduate Program, University of Calgary, Calgary, AB, Canada.
Michael L SamsomBiomedical Engineering Graduate Program, University of Calgary, Calgary, AB, Canada.
Suresh C RegmiFaculty of Kinesiology, KNB 426, 2500 University Dr. NW, University of Calgary, Calgary, AB, T2N 1N4, Canada.
Bridgett L SteeleFaculty of Kinesiology, KNB 426, 2500 University Dr. NW, University of Calgary, Calgary, AB, T2N 1N4, Canada.
Tannin A SchmidtBiomedical Engineering Graduate Program, University of Calgary, Calgary, AB, Canada. tschmidt@ucalgary.ca.ORCID http://orcid.org/0000-0001-7140-254X
University of Calgary · CA

Funding

Natural Sciences and Engineering Research Council of Canada RGPIN-2014-06518
6 · The paper itself

Abstract

backgroundThe objective of this study was to use confocal fluorescence recovery after photobleaching (FRAP) to examine the specific and dose-dependent effect of proteoglycan 4 (PRG4) on hyaluronan (HA) solutions of different molecular weight; and assess the effect of reduction and alkylation (R/A) of PRG4 on its effects on HA solutions.

methodsConfocal FRAP was used to determine the diffusion coefficient of fluorescein isothiocyanate (FITC)-dextran tracer (D

resultsPRG4 at both 45 and 450 μg/ml slowed the diffusion of the FITC-dextran tracer for all concentrations of HA and caused a decrease in the apparent mesh size within the HA solution. This effect was specific to PRG4, not observed with BSA, but not dependent on its tertiary/quaternary structure as the effect remained after R/A of PRG4.

conclusionsThese results demonstrate that PRG4 can significantly alter the solution properties of HA; PRG4 essentially reduced the permeability of the HA network. This effect may be due to PRG4 entangling HA molecules through binding and/or HA crowding PRG4 molecules into a self-assembled network. Collectively these findings contribute to the understanding of PRG4 and HA interaction(s) in solution and therefore the function of SF in diarthroidal joints.

Indexed as

AnimalsCattleDose-Response Relationship, DrugFluorescence Recovery After PhotobleachingHyaluronic AcidMicroscopy, ConfocalPharmaceutical SolutionsProteoglycan 4ProteoglycansHyaluronic AcidPharmaceutical SolutionsPRG4 protein, humanProteoglycan 4ProteoglycansConfocal FRAPHyaluronanProteoglycan 4Solution networkTracer diffusion

Identifiers

PMID30808331
PMCPMC6391837
OpenAlexW2524258384

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.