ArticleBiomaterials2019
Poly(ethylene glycol)-crosslinked gelatin hydrogel substrates with conjugated bioactive peptides influence endothelial cell behavior.
Article in Biomaterials, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 69 citations in OpenAlex.
- Conjugation of Proangiogenic Peptide to Enhance a Soft Tissue Bioink.Journal of biomedical materials research. Part A · 2025Article
- Dynamic Hydrogels in Breast Tumor Models.Gels (Basel, Switzerland) · 2025Review
- Heparin and Gelatin Co-Functionalized Polyurethane Artificial Blood Vessel for Improving Anticoagulation and Biocompatibility.Bioengineering (Basel, Switzerland) · 2025Article
- Dual-Self-Crosslinking Effect of Alginate-Di-Aldehyde with Natural and Synthetic Co-Polymers as Injectable In Situ-Forming Biodegradable Hydrogel.Gels (Basel, Switzerland) · 2024Article
- Three-dimensional breast cancer tumor models based on natural hydrogels: a review.Journal of Zhejiang University. Science. B · 2024Review
- Endothelial progenitor cells for fabrication of engineered vascular units and angiogenesis induction.Cell proliferation · 2024Review
- Synergy between Laminin-Derived Elastin-like Polypeptides (LELPs) Optimizes Cell Spreading.Biomacromolecules · 2024Article
- Harnessing the potential of hydrogels for advanced therapeutic applications: current achievements and future directions.Signal transduction and targeted therapy · 2024Review
- Engineered Synthetic Matrices for Human Intestinal Organoid Culture and Therapeutic Delivery.Advanced materials (Deerfield Beach, Fla.) · 2024Review
- Utilization of Lyotropic Liquid Crystalline Gels for Chronic Wound Management.Gels (Basel, Switzerland) · 2023Review
- Mimicking Molecular Pathways in the Design of Smart Hydrogels for the Design of Vascularized Engineered Tissues.International journal of molecular sciences · 2023Review
- Peptide-Based Hydrogels: Template Materials for Tissue Engineering.Journal of functional biomaterials · 2023Review
- A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells.ACS applied materials & interfaces · 2023Article
- A 3D bioprinted tumor model fabricated with gelatin/sodium alginate/decellularized extracellular matrix bioink.International journal of bioprinting · 2023Article
- Cells, growth factors and biomaterials used in tissue engineering for hair follicles regeneration.Regenerative therapy · 2022Review
- Synergistic osteogenic and angiogenic effects of KP and QK peptides incorporated with an injectable and self-healing hydrogel for efficient bone regeneration.Bioactive materials · 2022Article
- Chemical strategies to engineer hydrogels for cell culture.Nature reviews. Chemistry · 2022Review
- Mammalian Cell Interaction with Periodic Surface Nanostructures.International journal of molecular sciences · 2022Article
- Supramolecular Peptide Nanofiber Hydrogels for Bone Tissue Engineering: From Multihierarchical Fabrications to Comprehensive Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Peptide Biomaterials for Tissue Regeneration.Frontiers in bioengineering and biotechnology · 2022Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
The basement membrane is a specialized extracellular matrix substrate responsible for support and maintenance of epithelial and endothelial structures. Engineered basement membrane-like hydrogel systems have the potential to advance understanding of cell-cell and cell-matrix interactions by allowing precise tuning of the substrate or matrix biochemical and biophysical properties. In this investigation, we developed tunable hydrogel substrates with conjugated bioactive peptides to modulate cell binding and growth factor signaling by endothelial cells. Hydrogels were formed by employing a poly(ethylene glycol) crosslinker to covalently crosslink gelatin polymers and simultaneously conjugate laminin-derived YIGSR peptides or vascular endothelial growth factor (VEGF)-mimetic QK peptides to the gelatin. Rheological characterization revealed rapid formation of hydrogels with similar stiffnesses across tested formulations, and swelling analysis demonstrated dependency on peptide and crosslinker concentrations in hydrogels. Levels of phosphorylated VEGF Receptor 2 in cells cultured on hydrogel substrates revealed that while human umbilical vein endothelial cells (HUVECs) responded to both soluble and conjugated forms of the QK peptide, conditionally-immortalized human glomerular endothelial cells (GEnCs) only responded to the conjugated presentation of the peptide. Furthermore, whereas HUVECs exhibited greatest upregulation in gene expression when cultured on YIGSR- and QK-conjugated hydrogel substrates after 5 days, GEnCs exhibited greatest upregulation when cultured on Matrigel control substrates at the same time point. These results indicate that conjugation of bioactive peptides to these hydrogel substrates significantly influenced endothelial cell behavior in cultures but with differential responses between HUVECs and GEnCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.