ArticleAsian Pacific journal of cancer prevention : APJCP2019
In Vitro Cytotoxic Activity of Clinacanthus nutans Leaf Extracts Against HeLa Cells
Article in Asian Pacific journal of cancer prevention : APJCP, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 26 citations in OpenAlex.
- Phytochemical Analysis and Apoptosis-Inducing Anticancer Activity of Buddleja polystachia Leaf Extract against HeLa Cell Lines.Asian Pacific journal of cancer prevention : APJCP · 2026Article
- In Vitro Anticancer Activities of Senna singueana Leaf Extracts against HeLa Cell Lines.Asian Pacific journal of cancer prevention : APJCP · 2025Article
- Fractionation, identification of chemical constituents, and biological properties of cashew (Food science & nutrition · 2023Article
- A Narrative Review on the Phytochemistry, Pharmacology and Therapeutic Potentials ofMolecules (Basel, Switzerland) · 2021Review
- Dichloromethane fraction of Moringa oleifera leaf methanolic extract selectively inhibits breast cancer cells (MCF7) by induction of apoptosis via upregulation of Bax, p53 and caspase 8 expressions.Molecular biology reports · 2021Article
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- Recent Advancement in Anticancer Activity ofEvidence-based complementary and alternative medicine : eCAM · 2021Review
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- Antioxidant Effects, Antiproliferative Effects, and Molecular Docking ofMolecules (Basel, Switzerland) · 2020Article
- Supercritical CO₂ extraction ofJournal of advanced pharmaceutical technology & researchArticle
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Objective: This study was conducted to investigate the antiproliferative activity of extracts of Clinacanthus nutans leaves against human cervical cancer (HeLa) cells. Methods: C. nutans leaves were subjected to extraction using 80% methanol or water. The methanol extract was further extracted to obtain hexane, dichloromethane (DCM), and aqueous fractions. The antiproliferative activity of the extracts against HeLa cells was determined. The most cytotoxic extract was furthered analyzed by apoptosis and cell cycle assays, and the phytochemical constituents were screened by gas chromatography-mass spectrometry (GC-MS). Results: All of the extracts were antiproliferative against HeLa cells, and the DCM fraction had the lowest IC50 value of 70 μg/mL at 48 h. Microscopic studies showed that HeLa cells exposed to the DCM fraction exhibited marked morphological features of apoptosis. The flow cytometry study also confirmed that the DCM fraction induced apoptosis in HeLa cells, with cell cycle arrest at the S phase. GC-MS analysis revealed the presence of at least 28 compounds in the DCM fraction, most of which were fatty acids. Conclusion: The DCM fraction obtained using the extraction method described herein had a lower IC50 value than those reported in previous studies that characterized the anticancer activity of C. nutans against HeLa cells.
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