Evidence map›Paper›PMID 30805387›Full record

ArticleIranian journal of biotechnology2018

Functions of the Heterologous Intron-Derived Fragments Intra and Extra Factor IX-cDNA Coding Region on the Human Factor IX Expression in HepG2 and Hek-293T Cells.

Mohammad Reza Sam, Alireza Zomorodipour, Aliakbar Haddad-Mashadrizeh, Mahdi Ghorbani, Gholam Ali Kardar, Sohrab Sam

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Article in Iranian journal of biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Mohammad Reza SamDepartment of Cellular and Molecular Biotechnology, Institute of Biotechnology, Urmia University, Urmia, Iran.
Alireza ZomorodipourDepartment of Molecular Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Aliakbar Haddad-MashadrizehDepartment of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Mahdi GhorbaniDepartment of Molecular Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Gholam Ali KardarImmunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
Sohrab SamDepartment of Cellular and Molecular Biotechnology, Institute of Biotechnology, Urmia University, Urmia, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHuman FIX (hFIX) gene transfer into hepatocytes has provided a novel approach for treatment of hemophilia B. To obtain an improved expression of hFIX, the functional

objectivesWe aim to evaluate the functions of the heterologous intron-derived fragments intra and extra hFIX-cDNA coding region with respect to the hFIX expression in the hepatocytes and kidney cells. MATERIALS AND

methodsHepG2 cells as differentiated hepatocytes and Hek-293T cells as embryonic kidney cells were transfected with the different

resultsOur data indicate that the intron-less construct and

conclusionsThe embryonic kidney cells in addition to the differentiated hepatic cell lines could be successfully targeted by plasmid vectors. The intron-less and

Indexed as

Aldolase B intronic enhancerBeta-globin intronsHemophilia BHepatocyteHuman factor IX (hFIX)Plasmid

Identifiers

PMID30805387
PMCPMC6371630

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