Evidence map›Paper›PMID 30804650›Full record

ArticlePhysiology and molecular biology of plants : an international journal of functional plant biology2019

Screening internal controls for expression analyses involving numerous treatments by combining statistical methods with reference gene selection tools.

Joyous T Joseph, Najya Jabeen Poolakkalody, Jasmine M Shah

Abstract read
In one paragraph

Article in Physiology and molecular biology of plants : an international journal of functional plant biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Identification of a suitable method of inoculation for reducing background effect in mock-inoculated controls during gene expression studies inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2023
    Article
  3. Article
  4. Gene Expression Analysis by Quantitative Real-Time PCR for Floral Tissues.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  5. Essential role of theBiotechnologia · 2023
    Article
  6. Article
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Joyous T JosephDepartment of Plant Science, Central University of Kerala, Periye, Kasaragod, 671316 India.
Najya Jabeen PoolakkalodyDepartment of Plant Science, Central University of Kerala, Periye, Kasaragod, 671316 India.
Jasmine M ShahDepartment of Plant Science, Central University of Kerala, Periye, Kasaragod, 671316 India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Real-time PCR is always the method of choice for expression analyses involving comparison of a large number of treatments. It is also the favored method for final confirmation of transcript levels followed by high throughput methods such as RNA sequencing and microarray. Our analysis comprised 16 different permutation and combinations of treatments involving four different

Indexed as

AgrobacteriumArabidopsisNormalizationReal-time PCRReference genesStable expression

Identifiers

PMID30804650
PMCPMC6352529

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.