Evidence map›Paper›PMID 30804481›Full record

ArticleNature nanotechnology2019

High aspect ratio nanomaterials enable delivery of functional genetic material without DNA integration in mature plants.

Gozde S Demirer, Huan Zhang, Juliana L Matos, Natalie S Goh, Francis J Cunningham, Younghun Sung, Roger Chang, Abhishek J Aditham, Linda Chio, Myeong-Je Cho and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature nanotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 213 papers.

0numbers the graph read from it
0cells of the map it votes in
213citing papers in PubMed
51.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

213 citing papers in PubMed, 607 citations in OpenAlex.

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153 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Gozde S DemirerDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA, USA.
Huan ZhangDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA, USA.
Juliana L MatosDepartment of Plant and Microbial Biology, University of California, Berkeley, CA, USA.
Natalie S GohDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA, USA.
Francis J CunninghamDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA, USA.
Younghun SungDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA, USA.
Roger ChangDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA, USA.
Abhishek J AdithamDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA, USA.
Linda ChioDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA, USA.
Myeong-Je ChoInnovative Genomics Institute (IGI), Berkeley, CA, USA.
Brian StaskawiczDepartment of Plant and Microbial Biology, University of California, Berkeley, CA, USA.
Markita P LandryDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA, USA. landry@berkeley.edu.ORCID http://orcid.org/0000-0002-5832-8522
University of California, Berkeley · USInnovative Genomics Institute · USQB3 · US

Funding

Single-Molecule Imaging of Biological Trauma:Cytokine-Based Intracellular CommunicationR35GM128922 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI LANDRY, MARKITA · 2019 to 2023
$2.0M
A Zeiss Elyra S1 Structured Illumination Microscope for the Biological Imaging FaS10OD018136 · OD · UNIVERSITY OF CALIFORNIA BERKELEY · PI RUZIN, STEVEN EARL · 2014 to 2014
$600k
NIGMS NIH HHS R35 GM128922NIH HHS S10 OD018136
6 · The paper itself

Abstract

Genetic engineering of plants is at the core of sustainability efforts, natural product synthesis and crop engineering. The plant cell wall is a barrier that limits the ease and throughput of exogenous biomolecule delivery to plants. Current delivery methods either suffer from host-range limitations, low transformation efficiencies, tissue damage or unavoidable DNA integration into the host genome. Here, we demonstrate efficient diffusion-based biomolecule delivery into intact plants of several species with pristine and chemically functionalized high aspect ratio nanomaterials. Efficient DNA delivery and strong protein expression without transgene integration is accomplished in Nicotiana benthamiana (Nb), Eruca sativa (arugula), Triticum aestivum (wheat) and Gossypium hirsutum (cotton) leaves and arugula protoplasts. We find that nanomaterials not only facilitate biomolecule transport into plant cells but also protect polynucleotides from nuclease degradation. Our work provides a tool for species-independent and passive delivery of genetic material, without transgene integration, into plant cells for diverse biotechnology applications.

Indexed as

Gene Transfer TechniquesTransgenesGossypiumNicotianaPlants, Genetically ModifiedProtoplastsTriticum

Identifiers

PMID30804481
PMCPMC10461892
OpenAlexW2950849557

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.