ArticleScientific reports2019
Comprehensive identification of the full-length transcripts and alternative splicing related to the secondary metabolism pathways in the tea plant (Camellia sinensis).
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 67 citations in OpenAlex.
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- The landscape of fusion transcripts in plants: a new insight into genome complexity.BMC plant biology · 2024Article
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- Molecular Mechanism of Resistance toInternational journal of molecular sciences · 2024Article
- Tea-Derived Polyphenols Enhance Drought Resistance of Tea Plants (International journal of molecular sciences · 2024Article
- Comparative Transcriptome Profiling of CMS-D2 and CMS-D8 Systems Characterizes Fertility Restoration Genes Network in Upland Cotton.International journal of molecular sciences · 2023Article
- De novo full length transcriptome analysis of a naturally caffeine-free tea plant reveals specificity in secondary metabolic regulation.Scientific reports · 2023Article
- Alternative splicing in ABA signaling during seed germination.Frontiers in plant science · 2023Review
- Combination of long-read and short-read sequencing provides comprehensive transcriptome and new insight for Chrysanthemum morifolium ray-floret colorization.Scientific reports · 2022Article
- A high-resolution single-molecule sequencing-based Arabidopsis transcriptome using novel methods of Iso-seq analysis.Genome biology · 2022Article
- A Global Analysis of Alternative Splicing ofCurrent genomics · 2022Article
- Alternative Splicing and Its Roles in Plant Metabolism.International journal of molecular sciences · 2022Review
- Full-length transcriptome sequencing and comparative transcriptome analysis ofFrontiers in cellular and infection microbiology · 2022Article
- Transcriptomic Complexity in Strawberry Fruit Development and Maturation Revealed by Nanopore Sequencing.Frontiers in plant science · 2022Article
- United States tea: A synopsis of ongoing tea research and solutions to United States tea production issues.Frontiers in plant science · 2022Review
- EST-SSR Marker Development and Full-Length Transcriptome Sequence Analysis of Tiger Lily (Applied bionics and biomechanics · 2022Article
- Full-length transcriptome analysis of pecan (Carya illinoinensis) kernels.G3 (Bethesda, Md.) · 2021Article
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8 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Flavonoids, theanine and caffeine are the main secondary metabolites of the tea plant (Camellia sinensis), which account for the tea's unique flavor quality and health benefits. The biosynthesis pathways of these metabolites have been extensively studied at the transcriptional level, but the regulatory mechanisms are still unclear. In this study, to explore the transcriptome diversity and complexity of tea plant, PacBio Iso-Seq and RNA-seq analysis were combined to obtain full-length transcripts and to profile the changes in gene expression during the leaf development. A total of 1,388,066 reads of insert (ROI) were generated with an average length of 1,762 bp, and more than 54% (755,716) of the ROIs were full-length non-chimeric (FLNC) reads. The Benchmarking Universal Single-Copy Orthologue (BUSCO) completeness was 92.7%. A total of 93,883 non-redundant transcripts were obtained, and 87,395 (93.1%) were new alternatively spliced isoforms. Meanwhile, 7,650 differential expression transcripts (DETs) were identified. A total of 28,980 alternative splicing (AS) events were predicted, including 1,297 differential AS (DAS) events. The transcript isoforms of the key genes involved in the flavonoid, theanine and caffeine biosynthesis pathways were characterized. Additionally, 5,777 fusion transcripts and 9,052 long non-coding RNAs (lncRNAs) were also predicted. Our results revealed that AS potentially plays a crucial role in the regulation of the secondary metabolism of the tea plant. These findings enhanced our understanding of the complexity of the secondary metabolic regulation of tea plants and provided a basis for the subsequent exploration of the regulatory mechanisms of flavonoid, theanine and caffeine biosynthesis in tea plants.
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