ArticleNature communications2019
Assembly and functionality of the ribosome with tethered subunits.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Article
- Article
- Saskemycin, a potent and selective antimycobacterial agent targeting a unique site on the ribosome.Research square · 2025Article
- Evolution of ribosome X-ray crystallography: from first crystals to atomic resolution.Biophysical reviews · 2025Review
- Genetic Code Expansion: Recent Developments and Emerging Applications.Chemical reviews · 2025Review
- Autonomous ribosome biogenesis in vitro.Nature communications · 2025Article
- Cracking the Code: Reprogramming the Genetic Script in Prokaryotes and Eukaryotes to Harness the Power of Noncanonical Amino Acids.Chemical reviews · 2024Review
- Ribosome Pool Engineering Increases Protein Biosynthesis Yields.ACS central science · 2024Article
- Mapping the in vivo fitness landscape of a tethered ribosome.Science advances · 2023Article
- Cell-free Biosynthesis of Peptidomimetics.Biotechnology and bioprocess engineering : BBE · 2023Review
- Applying a Gene Reversal Rate Computational Methodology to Identify Drugs for a Rare Cancer: Inflammatory Breast Cancer.Cancer informatics · 2023Article
- Context-based sensing of orthosomycin antibiotics by the translating ribosome.Nature chemical biology · 2022Article
- Mobile Translation Systems Generate Genomically EngineeredACS synthetic biology · 2022Article
- Three-dimensional structure-guided evolution of a ribosome with tethered subunits.Nature chemical biology · 2022Article
- Article
- Ribosome-Directed Therapies in Cancer.Biomedicines · 2022Review
- A molecular map of long non-coding RNA expression, isoform switching and alternative splicing in osteoarthritis.Human molecular genetics · 2022Article
- Plasticity and conditional essentiality of modification enzymes for domain V ofRNA (New York, N.Y.) · 2022Article
- Specialised ribosomes as versatile regulators of gene expression.RNA biology · 2022Review
- Directed evolution of rRNA improves translation kinetics and recombinant protein yield.Nature communications · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Ribo-T is an engineered ribosome whose small and large subunits are tethered together by linking 16S rRNA and 23S rRNA in a single molecule. Although Ribo-T can support cell proliferation in the absence of wild type ribosomes, Ribo-T cells grow slower than those with wild type ribosomes. Here, we show that cell growth defect is likely explained primarily by slow Ribo-T assembly rather than its imperfect functionality. Ribo-T maturation is stalled at a late assembly stage. Several post-transcriptional rRNA modifications and some ribosomal proteins are underrepresented in the accumulated assembly intermediates and rRNA ends are incompletely trimmed. Ribosome profiling of Ribo-T cells shows no defects in translation elongation but reveals somewhat higher occupancy by Ribo-T of the start codons and to a lesser extent stop codons, suggesting that subunit tethering mildly affects the initiation and termination stages of translation. Understanding limitations of Ribo-T system offers ways for its future development.
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